ERF (NM_001301035) Human Untagged Clone

CAT#: SC336326

ERF (untagged) - Human Ets2 repressor factor (ERF), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERF
• Synonyms: CHYTS; CRS4; PE-2; PE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001301035, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATTACGACAAGCTGAGCCGGGCCCTGCGCTATTACTATAACAAGCGCATTCTGCACAAGACCAAGG
  GGAAACGGTTCACCTACAAGTTCAATTTCAACAAACTGGTGCTGGTCAATTACCCATTCATTGATGTGGG
  GTTGGCTGGGGGTGCAGTGCCCCAGAGTGCCCCGCCAGTGCCGTCGGGTGGTAGCCACTTCCGCTTCCCT
  CCCTCAACGCCCTCCGAGGTGCTGTCCCCCACCGAGGACCCCCGCTCACCACCAGCCTGCTCTTCATCTT
  CATCTTCCCTCTTCTCGGCTGTGGTGGCCCGCCGCCTGGGCCGAGGCTCAGTCAGTGACTGTAGTGATGG
  CACGTCAGAGCTGGAGGAACCGCTGGGAGAGGATCCCCGCGCCCGACCACCCGGCCCTCCGGATCTGGGT
  GCCTTCCGAGGGCCCCCGCTGGCCCGCCTGCCCCATGACCCTGGTGTCTTCCGAGTCTATCCCCGGCCTC
  GGGGTGGCCCTGAACCCCTCAGCCCCTTCCCTGTGTCGCCTCTGGCCGGTCCTGGATCCCTGCTGCCCCC
  TCAGCTCTCCCCGGCTCTGCCCATGACGCCCACCCACCTGGCCTACACTCCCTCGCCCACGCTGAGCCCG
  ATGTACCCCAGTGGTGGCGGGGGGCCCAGCGGCTCAGGGGGAGGCTCCCACTTCTCCTTCAGCCCTGAGG
  ACATGAAACGGTACCTGCAGGCCCACACCCAAAGCGTCTACAACTACCACCTCAGCCCCCGCGCCTTCCT
  GCACTACCCTGGGCTGGTGGTGCCCCAGCCCCAGCGCCCTGACAAGTGCCCGCTGCCGCCCATGGCACCC
  GAGACCCCACCGGTCCCCTCCTCGGCCTCGTCATCCTCTTCTTCTTCTTCCTCCCCATTCAAGTTTAAGC
  TCCAGCCGCCCCCACTCGGACGCCGGCAGCGGGCAGCTGGGGAGAAGGCCGTAGCCGGTGCTGACAAGAG
  CGGTGGCAGTGCAGGCGGGCTGGCTGAGGGGGCAGGGGCGCTAGCCCCACCGCCCCCGCCACCACAGATC
  AAGGTGGAGCCCATCTCGGAAGGCGAGTCGGAGGAGGTAGAGGTGACTGACATCAGTGATGAGGATGAGG
  AAGACGGGGAGGTGTTCAAGACGCCCCGTGCCCCACCTGCACCCCCTAAGCCTGAGCCCGGCGAGGCACC
  CGGGGCATCCCAGTGCATGCCCCTCAAGCTACGCTTTAAGCGGCGCTGGAGTGAAGACTGTCGCCTCGAA
  GGGGGTGGGGGCCCCGCTGGGGGCTTTGAGGATGAGGGTGAGGACAAGAAGGTGCGTGGGGAGGGGCCTG
  GGGAGGCTGGGGGGCCCCTCACCCCAAGGCGGGTGAGCTCTGACCTCCAGCATGCCACGGCCCAGCTCTC
  CCTGGAGCACCGAGACTCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301035
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301035.1, NP_001287964.1
• RefSeq Size: 2634 bp
• RefSeq ORF: 1422 bp
• Locus ID: 2077
• Cytogenetics: 19q13.2
• Protein Families: Transcription Factors
• Gene Summary: 'ETS2 is a transcription factor and protooncogene involved in development, apoptosis, and the regulation of telomerase. The protein encoded by this gene binds to the ETS2 promoter and is a strong repressor of ETS2 transcription. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]'
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Variants 2, 3, and 4 all encode isoform 2.