PDE1B (NM_001288769) Human Untagged Clone

CAT#: SC336453

PDE1B (untagged) - Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE1B
• Synonyms: HEL-S-79p; PDE1B1; PDES1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001288769, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGAAGCAGTTGGAGAATGGGGAGATAAACATTGAGGAGCTGAAGAAAAATCTGGAGTACACAGCTT
  CTCTGCTGGAAGCCGTCTACATAGATGAGACACGGCAAATCTTGGACACGGAGGACGAGCTGCAGGAGCT
  GCGGTCAGATGCCGTGCCTTCGGAGGTGCGGGACTGGCTGGCCTCCACCTTCACCCAGCAGGCCCGGGCC
  AAAGGCCGCCGAGCAGAGGAGAAGCCCAAGTTCCGAAGCATTGTGCACGCTGTGCAGGCTGGGATCTTCG
  TGGAACGGATGTTCCGGAGAACATACACCTCTGTGGGCCCCACTTACTCTACTGCGGTTCTCAACTGTCT
  CAAGAACCTGGATCTCTGGTGCTTTGATGTCTTTTCCTTGAACCAGGCAGCAGATGACCATGCCCTGAGG
  ACCATTGTTTTTGAGTTGCTGACTCGGCATAACCTCATCAGCCGCTTCAAGATTCCCACTGTGTTTTTGA
  TGAGTTTCCTGGATGCCTTGGAGACAGGCTATGGGAAGTACAAGAATCCTTACCACAACCAGATCCACGC
  AGCCGATGTTACCCAGACAGTCCATTGCTTCTTGCTCCGCACAGGGATGGTGCACTGCCTGTCGGAGATT
  GAGCTCCTGGCCATCATCTTTGCTGCAGCTATCCATGATTATGAGCACACGGGCACTACCAACAGCTTCC
  ACATCCAGACCAAGTCAGAATGTGCCATCGTGTACAATGATCGTTCAGTGCTGGAGAATCACCACATCAG
  CTCTGTTTTCCGATTGATGCAGGATGATGAGATGAACATTTTCATCAACCTCACCAAGGATGAGTTTGTA
  GAACTCCGAGCCCTGGTCATTGAGATGGTGTTGGCCACAGACATGTCCTGCCATTTCCAGCAAGTGAAGA
  CCATGAAGACAGCCTTGCAACAGCTGGAGAGGATTGACAAGCCCAAGGCCCTGTCTCTACTGCTCCATGC
  TGCTGACATCAGCCACCCAACCAAGCAGTGGTTGGTCCACAGCCGTTGGACCAAGGCCCTCATGGAGGAA
  TTCTTCCGTCAGGGTGACAAGGAGGCAGAGTTGGGCCTGCCCTTTTCTCCACTCTGTGACCGCACTTCCA
  CTCTAGTGGCACAGTCTCAGATAGGGTTCATCGACTTCATTGTGGAGCCCACATTCTCTGTGCTGACTGA
  CGTGGCAGAGAAGAGTGTTCAGCCCCTGGCGGATGAGGACTCCAAGTCTAAAAACCAGCCCAGCTTTCAG
  TGGCGCCAGCCCTCTCTGGATGTGGAAGTGGGAGACCCCAACCCTGATGTGGTCAGCTTTCGTTCCACCT
  GGGTCAAGCGCATTCAGGAGAATAAGCAGAAATGGAAGGAACGGGCAGCAAGTGGCATCACCAACCAGAT
  GTCCATTGACGAGCTGTCCCCCTGTGAAGAAGAGGCCCCCCCATCCCCTGCCGAAGATGAACACAACCAG
  AATGGGAATCTGGATTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001288769
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288769.1, NP_001275698.1
• RefSeq Size: 3083 bp
• RefSeq ORF: 1488 bp
• Locus ID: 5153
• Cytogenetics: 12q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (4) contains a distinct 5' UTR, lacks part of the 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, compared to isoform 1.