BTD (NM_001281725) Human Untagged Clone

CAT#: SC336605

BTD (untagged) - Human biotinidase (BTD), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BTD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001281725, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGGAGCCAGAAGTAAGCTTGCTCTTTTCCTCTGCGGCTGTTACGTGGTTGCCCTGGGAGCCCACA
  CCGGGGAGGAGAGCGTGGCTGACCATCACGAGGCTGAATATTATGTGGCTGCCGTGTATGAGCATCCATC
  CATCCTGAGTCTGAACCCTCTGGCTCTCATCAGCCGCCAAGAGGCCTTGGAGCTCATGAACCAGAACCTT
  GACATCTATGAACAGCAAGTGATGACTGCAGCCCAAAAGGATGTACAGATTATAGTGTTTCCAGAAGATG
  GCATTCATGGATTCAACTTTACAAGAACATCCATTTATCCATTTTTGGACTTCATGCCGTCTCCCCAGGT
  GGTCAGGTGGAACCCATGCCTGGAGCCTCACCGCTTCAATGACACAGAGGTGCTCCAGCGCCTGAGTTGT
  ATGGCCATCAGGGGAGATATGTTCTTGGTGGCCAATCTTGGGACAAAGGAGCCTTGTCATAGCAGTGACC
  CAAGGTGCCCAAAAGATGGGAGATACCAGTTCAACACAAATGTCGTGTTCAGCAATAATGGAACCCTTGT
  TGACCGCTACCGTAAACACAACCTCTACTTTGAGGCAGCATTCGATGTTCCTCTTAAAGTGGATCTCATC
  ACCTTTGATACCCCCTTTGCTGGCAGGTTTGGCATCTTCACATGCTTTGATATATTGTTCTTTGACCCTG
  CCATCAGAGTCCTCAGAGACTACAAGGTGAAGCATGTTGTGTACCCAACTGCCTGGATGAACCAGCTCCC
  ACTCTTGGCAGCAATTGAGATTCAGAAAGCTTTTGCTGTTGCCTTTGGCATCAACGTTCTGGCAGCTAAT
  GTCCACCACCCAGTTCTGGGGATGACAGGAAGTGGCATACACACCCCTCTGGAGTCCTTTTGGTACCATG
  ACATGGAAAATCCCAAAAGTCACCTTATAATTGCCCAGGTGGCCAAAAATCCAGTGGGTCTCATTGGTGC
  AGAGAATGCAACAGGTGAAACGGACCCATCCCATAGTAAGTTTTTAAAAATTTTGTCAGGCGATCCGTAC
  TGTGAGAAGGATGCTCAGGAAGTCCACTGTGATGAGGCCACCAAGTGGAACGTGAATGCTCCTCCCACAT
  TTCACTCTGAGATGATGTATGACAATTTCACCCTGGTCCCTGTCTGGGGAAAGGAAGGCTATCTCCACGT
  CTGTTCCAATGGCCTCTGCTGTTATTTACTTTACGAGAGGCCCACCTTATCCAAAGAGCTGTATGCCCTG
  GGGGTCTTTGATGGGCTTCACACAGTACATGGCACTTACTACATCCAAGTGTGTGCCCTGGTCAGGTGTG
  GGGGTCTTGGCTTCGACACCTGTGGACAGGAAATCACAGAGGCCACGGGGATATTTGAGTTTCACCTGTG
  GGGCAACTTCAGTACTTCCTATATCTTTCCTTTGTTTCTGACCTCAGGGATGACCCTAGAAGTCCCTGAC
  CAGCTTGGCTGGGAGAATGACCACTATTTCCTGAGGAAAAGTAGGCTGTCCTCTGGGCTGGTGACGGCGG
  CTCTCTATGGGCGCTTGTATGAGAGGGACTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281725
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281725.2, NP_001268654.1
• RefSeq Size: 4092 bp
• RefSeq ORF: 1572 bp
• Locus ID: 686
• Cytogenetics: 3p25.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Biotin metabolism, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1-4 and 6 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.