MYH (MUTYH) (NM_001293190) Human Untagged Clone

CAT#: SC336675

MUTYH (untagged) - Human mutY homolog (MUTYH), transcript variant alpha2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MUTYH
• Synonyms: MYH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001293190, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACACCGCTCGTCTCCCGCCTGAGTCGTCTGTGGGCCATCATGAGGAAGCCACGAGCAGCCGTGGGAA
  GTGGTCACAGGAAGCAGGCAGCCAGCCAGGAAGGGAGGCAGAAGCATGCTAAGAACAACAGTCAGGCCAA
  GCCTTCTGCCTGTGATGCAGGCCTGGCCAGGCAGCCGGAAGAGGTGGTATTGCAGGCCTCTGTCTCCTCA
  TACCATCTATTCAGAGACGTAGCTGAAGTCACAGCCTTCCGAGGGAGCCTGCTAAGCTGGTACGACCAAG
  AGAAACGGGACCTACCATGGAGAAGACGGGCAGAAGATGAGATGGACCTGGACAGGCGGGCATATGCTGT
  GTGGGTCTCAGAGGTCATGCTGCAGCAGACCCAGGTTGCCACTGTGATCAACTACTATACCGGATGGATG
  CAGAAGTGGCCTACACTGCAGGACCTGGCCAGTGCTTCCCTGGAGGAGGTGAATCAACTCTGGGCTGGCC
  TGGGCTACTATTCTCGTGGCCGGCGGCTGCAGGAGGGAGCTCGGAAGGTGGTAGAGGAGCTAGGGGGCCA
  CATGCCACGTACAGCAGAGACCCTGCAGCAGCTCCTGCCTGGCGTGGGGCGCTACACAGCTGGGGCCATT
  GCCTCTATCGCCTTTGGCCAGGCAACCGGTGTGGTGGATGGCAACGTAGCACGGGTGCTGTGCCGTGTCC
  GAGCCATTGGTGCTGATCCCAGCAGCACCCTTGTTTCCCAGCAGCTCTGGGGTCTAGCCCAGCAGCTGGT
  GGACCCAGCCCGGCCAGGAGATTTCAACCAAGCAGCCATGGAGCTAGGGGCCACAGTGTGTACCCCACAG
  CGCCCACTGTGCAGCCAGTGCCCTGTGGAGAGCCTGTGCCGGGCACGCCAGAGAGTGGAGCAGGAACAGC
  TCTTAGCCTCAGGGAGCCTGTCGGGCAGTCCTGACGTGGAGGAGTGTGCTCCCAACACTGGACAGTGCCA
  CCTGTGCCTGCCTCCCTCGGAGCCCTGGGACCAGACCCTGGGAGTGGTCAACTTCCCCAGAAAGGCCAGC
  CGCAAGCCCCCCAGGGAGGAGAGCTCTGCCACCTGTGTTCTGGAACAGCCTGGGGCCCTTGGGGCCCAAA
  TTCTGCTGGTGCAGAGGCCCAACTCAGGTCTGCTGGCAGGACTGTGGGAGTTCCCGTCCGTGACCTGGGA
  GCCCTCAGAGCAGCTTCAGCGCAAGGCCCTGCTGCAGGAACTACAGCGTTGGGCTGGGCCCCTCCCAGCC
  ACGCACCTCCGGCACCTTGGGGAGGTTGTCCACACCTTCTCTCACATCAAGCTGACATATCAAGTATATG
  GGCTGGCCTTGGAAGGGCAGACCCCAGTGACCACCGTACCACCAGGTGCTCGCTGGCTGACGCAGGAGGA
  ATTTCACACCGCAGCTGTTTCCACCGCCATGAAAAAGGTTTTCCGTGTGTATCAGGGCCAACAGCCAGGG
  ACCTGTATGGGTTCCAAAAGGTCCCAGGTGTCCTCTCCGTGCAGTCGGAAAAAGCCCCGCATGGGCCAGC
  AAGTCCTGGATAATTTCTTTCGGTCTCACATCTCCACTGATGCACACAGCCTCAACAGTGCAGCCCAGTG
  A
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293190
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293190.1, NP_001280119.1
• RefSeq Size: 1906 bp
• RefSeq ORF: 1611 bp
• Locus ID: 4595
• Cytogenetics: 1p34.1
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair
• Gene Summary: 'This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]'
  Transcript Variant: This variant (alpha2) uses an alternate in-frame splice site in the 5' coding region, compared to variant alpha5. The resulting isoform (6) lacks an internal segment, compared to isoform 5.