EYA3 (NM_001282560) Human Untagged Clone

CAT#: SC336679

EYA3 (untagged) - Human EYA transcriptional coactivator and phosphatase 3 (EYA3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EYA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001282560, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGAAGAGCAAGATTTACCAGAGCAACCAGTGAAAAAAGCCAAGATGCAGGAATCAGGAGAGCAAA
  CTATAAGTCAAGTAAGCAATCCAGATGTCAGTGATCAGAAGCCTGAAACATCAAGCCTTGCTTCAAACCT
  TCCCATGTCAGAGGAAATTATGACATGCACCGATTACATCCCTCGCTCATCCAATGATTATACCTCACAA
  ATGTATTCTGCAAAACCTTATGCACATATTCTCTCAGTTCCTGTTTCGGAAACTGCTTACCCTGGACAGA
  CTCAATACCAGACACTACAGCAGACTCAACCCTATGCTGTCTACCCTCAGGCAACCCAAACGTATGGACT
  ACCTCCTTTTGCTTCAAGCACAAATGCCAGCCTGATATCTACTTCTTCTACAATTGCCAATATTCCAGCA
  GCAGCAGTAGCCAGCATCTCAAACCAGGATTATCCCACCTATACTATTCTTGGTCAGAATCAGTACCAGG
  CCTGCTACCCCAGCTCCAGCTTTGGAGTCACAGGTCAGACTAACAGTGATGCAGAGAGCACCACATTAGC
  AGCAACCACATACCAGTCGGAGAAGCCTAGTGTCATGGCGCCTGCACCTGCAGCACAGAGACTTTCCTCT
  GGAGACCCTTCTACAAGTCCATCTTTGTCCCAGACTACACCAAGTAAAGATACTGATGATCAGTCCAGGA
  AAAACATGACTAGCAAGAACCGGGGCAAGAGGAAAGCTGATGCCACTTCTTCCCAAGACAGTGAATTAGA
  ACGGGTATTTCTGTGGGACTTGGATGAAACCATCATCATCTTCCACTCACTTCTTACTGGATCCTATGCC
  CAGAAATATGGAAAGGACCCAACAGTAGTGATTGGCTCAGGTTTAACAATGGAAGAAATGATTTTTGAAG
  TGGCTGATACTCATCTATTTTTCAATGACTTAGAGGAGTGTGACCAGGTACATGTGGAAGATGTGGCTTC
  TGATGACAATGGCCAAGACTTGAGCAACTACAGTTTCTCAACAGATGGTTTCAGTGGCTCAGGAGGTAGT
  GGCAGCCATGGTTCATCTGTGGGTGTTCAGGGAGGTGTGGACTGGATGAGGAAACTAGCTTTCCGCTACC
  GGAAAGTGAGAGAAATCTATGATAAGCATAAAAGCAACGTGGGTGGTCTCCTCAGTCCCCAGAGGAAGGA
  AGCACTGCAGAGATTAAGAGCAGAAATTGAAGTTTTAACAGATTCCTGGTTAGGAACTGCATTAAAGTCC
  TTACTTCTCATCCAGTCCAGAAAGAATTGTGTGAATGTTCTGATCACTACCACCCAGCTGGTTCCAGCCC
  TGGCCAAGGTTCTCCTATATGGACTAGGAGAAATATTTCCTATTGAGAACATCTATAGTGCTACCAAAAT
  TGGTAAGGAGAGCTGCTTTGAGAGAATTGTGTCAAGGTTTGGAAAGAAAGTCACATATGTAGTGATTGGA
  GATGGACGAGATGAAGAAATTGCAGCCAAACAGCAACTCTATTTCTTAGACATGGAGGCATTGGGTTGCC
  AGCTAGAACCAACTGCCCTGATACTCTTCATCCAGTTAAGTGGAAACCTTAGCAACTATAATAAACTATG
  A
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282560
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282560.1, NP_001269489.1
• RefSeq Size: 1823 bp
• RefSeq ORF: 1611 bp
• Locus ID: 2140
• Cytogenetics: 1p35.3
• Protein Families: Phosphatase, Transcription Factors
• Gene Summary: 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, and contains an alternate 3' terminal exon and thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a.