DNA polymerase eta (POLH) (NM_001291969) Human Untagged Clone

CAT#: SC336898

POLH (untagged) - Human polymerase (DNA directed), eta (POLH), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLH
• Synonyms: RAD30; RAD30A; XP-V; XPV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001291969, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGCTCGTGCATTTGGAGTCACTAGAAGTATGTGGGCAGATGATGCTAAGAAGTTATGTCCAGATCT
  TCTACTGGCACAAGTTCGTGAGTCCCGTGGGAAAGCTAACCTCACCAAAGGGGATGCGAAAACAAGGCTT
  ATTTCAATGGCTCGATTCTCTTCAGATTGATAACCTCACCTCTCCAGACCTGCAGCTCACCGTGGGAGCA
  GTGATTGTGGAGGAAATGAGAGCAGCCATAGAGAGGGAGACTGGTTTTCAGTGTTCAGCTGGAATTTCAC
  ACAATAAGGTCCTGGCAAAACTGGCCTGTGGACTAAACAAGCCCAACCGCCAAACCCTGGTTTCACATGG
  GTCAGTCCCACAGCTCTTCAGCCAAATGCCCATTCGCAAAATCCGTAGTCTTGGAGGAAAGCTAGGGGCC
  TCTGTCATTGAGATCCTAGGGATAGAATACATGGGTGAACTGACCCAGTTCACTGAATCCCAGCTCCAGA
  GTCATTTTGGGGAGAAGAATGGGTCTTGGCTATATGCCATGTGCCGAGGGATTGAACATGATCCAGTTAA
  ACCCAGGCAACTACCCAAAACCATTGGCTGTAGTAAGAACTTCCCAGGAAAAACAGCTCTTGCTACTCGG
  GAACAGGTACAATGGTGGCTGTTGCAATTAGCCCAGGAACTAGAGGAGAGACTGACTAAAGACCGAAATG
  ATAATGACAGGGTAGCCACCCAGCTGGTTGTGAGCATTCGTGTACAAGGAGACAAACGCCTCAGCAGCCT
  GCGCCGCTGCTGTGCCCTTACCCGCTATGATGCTCACAAGATGAGCCATGATGCATTTACTGTCATCAAG
  AACTGTAATACTTCTGGAATCCAGACAGAATGGTCTCCTCCTCTCACAATGCTTTTCCTCTGTGCTACAA
  AATTTTCTGCCTCTGCCCCTTCATCTTCTACAGACATCACCAGCTTCTTGAGCAGTGACCCAAGTTCTCT
  GCCAAAGGTGCCAGTTACCAGCTCAGAAGCTAAGACCCAGGGAAGTGGCCCAGCGGTGACAGCCACTAAG
  AAAGCAACCACGTCTCTGGAATCATTCTTCCAAAAAGCTGCAGAAAGGCAGAAAGTTAAAGAAGCTTCGC
  TTTCATCTCTTACTGCTCCCACTCAGGCTCCCATGAGCAATTCACCATCCAAGCCCTCATTACCTTTTCA
  AACCAGTCAAAGTACAGGAACTGAGCCCTTCTTTAAGCAGAAAAGTCTGCTTCTAAAGCAGAAACAGCTT
  AATAATTCTTCAGTTTCTTCCCCCCAACAAAACCCATGGTCCAACTGTAAAGCATTACCAAACTCTTTAC
  CAACAGAGTATCCAGGGTGTGTCCCTGTTTGTGAAGGGGTGTCGAAGCTAGAAGAATCCTCTAAAGCAAC
  TCCTGCAGAGATGGATTTGGCCCACAACAGCCAAAGCATGCACGCCTCTTCAGCTTCCAAATCTGTGCTG
  GAGGTGACTCAGAAAGCAACCCCAAATCCAAGTCTTCTAGCTGCTGAGGACCAAGTGCCCTGTGAGAAGT
  GTGGCTCCCTGGTACCGGTATGGGATATGCCAGAACACATGGACTATCATTTTGCATTGGAGTTGCAGAA
  ATCCTTTTTGCAGCCCCACTCTTCAAACCCCCAGGTTGTTTCTGCCGTATCTCATCAAGGCAAAAGAAAT
  CCCAAGAGCCCTTTGGCCTGCACTAATAAACGCCCCAGGCCTGAGGGCATGCAAACATTGGAATCATTTT
  TTAAGCCATTAACACATTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291969
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291969.1, NP_001278898.1
• RefSeq Size: 8053 bp
• RefSeq ORF: 1770 bp
• Locus ID: 5429
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (2) lacks two alternate exons compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.