Calpastatin (CAST) (NM_001284213) Human Untagged Clone

CAT#: SC337062

CAST (untagged) - Human calpastatin (CAST), transcript variant 14

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAST
• Synonyms: BS-17; PLACK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001284213, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATCCCACAGAAACCAAGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCTGTCTG
  TGGTTCATGAGAAAAAATCCCAAGAAGGAAAGCCAAAAGAACACACAGAGCCAAAAAGCCTACCCAAGCA
  GGCATCAGATACAGGAAGTAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCAGCTGAACAGCAGCCA
  TCAGAGAAATCAACAGAACCAAAGTCAGGCATGGATGCTGCTTTGGATGACTTAATAGATACTTTAGGAG
  GACCTGAAGAAACTGAAGAAGAAAATACAACGTATACTGGACCAGAAGTTTCAGATCCAATGAGTTCCAC
  CTACATAGAGGAATTGGGTAAAAGAGAAGTCACAATTCCTCCAAAATATAGGGAACTATTGGCTAAAAAG
  GAAGGGATCACAGGGCCTCCTGCAGACTCTTCGAAACCCATAGGGCCAGATGATGCTATAGACGCCTTGT
  CATCTGACTTCACCTGTGGGTCGCCTACAGCTGCTGGAAAGAAAACTGAAAAAGAGGAATCTACAGAAGT
  TTTAAAAGCTCAGTCAGCAGGGACAGTCAGAAGTGCTGCTCCACCCCAAGAGAAGAAAAGAAAGGTGGAG
  AAGGATACAATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCACTGGGCACCCGGCAAGCAGAACCTG
  AGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAGGCAAAAGCTAAAGAAGAAAAACTAGAGAAGTGTGG
  TGAGGATGATGAAACAATCCCATCTGAGTACAGATTAAAACCAGCCACGGATAAAGATGGAAAACCACTA
  TTGCCAGAGCCTGAAGAAAAACCCAAGCCTCGGAGTGAATCAGAACTCATTGATGAACTTTCAGAAGATT
  TTGACCGGTCTGAATGTAAAGAGAAACCATCTAAGCCAACTGAAAAGACAGAAGAATCTAAGGCCGCTGC
  TCCAGCTCCTGTGTCGGAGGCTGTGTGTCGGACCTCCATGTGTAGTATACAGTCAGCACCCCCTGAGCCG
  GCTACCTTGAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTGGCTGATAGCCTGGGGAAAAAGGAAG
  CAGATCCAGAAGATGGAAAACCTGTGATGGATAAAGTCAAGGAGAAGGCCAAAGAAGAAGACCGTGAAAA
  GCTTGGTGAAAAAGAAGAAACAATTCCTCCTGATTATAGATTAGAAGAGGTCAAGGATAAAGATGGAAAG
  CCACTCCTGCCAAAAGAGTCTAAGGAACAGCTTCCACCCATGAGTGAAGACTTCCTTCTGGATGCTTTGT
  CTGAGGACTTCTCTGGTCCACAAAATGCTTCATCTCTTAAATTTGAAGATGCTAAACTTGCTGCTGCCAT
  CTCTGAAGTGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCTGGAGCCCCACCCCGTGATACCTCGCAG
  AGTGACAAAGACCTCGATGATGCCTTGGATAAACTCTCTGACAGTCTAGGACAAAGGCAGCCTGACCCAG
  ATGAGAACAAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAAGCTGAACATAGAGACAAGCTTGGAGA
  AAGAGATGACACTATCCCACCTGAATACAGACATCTCCTGGATGATAATGGACAGGACAAACCAGTGAAG
  CCACCTACAAAGAAATCAGAGGATTCAAAGAAACCTGCAGATGACCAAGACCCCATTGATGCTCTCTCAG
  GAGATCTGGACAGCTGTCCCTCCACTACAGAAACCTCACAGAACACAGCAAAGGATAAGTGCAAGAAGGC
  TGCTTCCAGCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAGGATTCAGCAAAGACAACAGAGGAAACT
  TCCAAGCCAAAAGATGACTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284213
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284213.2, NP_001271142.1
• RefSeq Size: 4154 bp
• RefSeq ORF: 1911 bp
• Locus ID: 831
• Cytogenetics: 5q15
• Gene Summary: 'The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]'