PKC theta (PRKCQ) (NM_001282644) Human Untagged Clone

CAT#: SC337170

PRKCQ (untagged) - Human protein kinase C, theta (PRKCQ), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKCQ
• Synonyms: nPKC-theta; PRKCT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001282644, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGAATCAGAACGGGCAGATGTATATCCAGAAAAAGCCTACCATGTACCCACCCTGGGACAGCACTT
  TTGATGCCCATATCAACAAGGGAAGAGTCATGCAGATCATTGTGAAAGGCAAAAACGTGGACCTCATCTC
  TGAAACCACCGTGGAGCTCTACTCGCTGGCTGAGAGGTGCAGGAAGAACAACGGGAAGACAGAAATATGG
  TTAGAGCTGAAACCTCAAGGCCGAATGCTAATGAATGCAAGATACTTTCTGGAAATGAGTGACACAAAGG
  ACATGAATGAATTTGAGACGGAAGGCTTCTTTGCTTTGCATCAGCGCCGGGGTGCCATCAAGCAGGCAAA
  GGTCCACCACGTCAAGTGCCACGAGTTCACTGCCACCTTCTTCCCACAGCCCACATTTTGCTCTGTCTGC
  CACGAGTTTGTCTGGGGCCTGAACAAACAGGGCTACCAGTGCCGACAATGCAATGCAGCAATTCACAAGA
  AGTGTATTGATAAAGTTATAGCAAAGTGCACAGGATCAGCTATCAATAGCCGAGAAACCATGTTCCACAA
  GGAGAGATTCAAAATTGACATGCCACACAGATTTAAAGTCTACAATTACAAGAGCCCGACCTTCTGTGAA
  CACTGTGGGACCCTGCTGTGGGGACTGGCACGGCAAGGACTCAAGTGTGATGCATGTGGCATGAATGTGC
  ATCATAGATGCCAGACAAAGGTGGCCAACCTTTGTGGCATAAACCAGAAGCTAATGGCTGAAGCGCTGGC
  CATGATTGAGAGCACTCAACAGGCTCGCTGCTTAAGAGATACTGAACAGATCTTCAGAGAAGGTCCGGTT
  GAAATTGGTCTCCCATGCTCCATCAAAAATGAAGCAAGGCCGCCATGTTTACCGACACCGGGAAAAAGAG
  AGCCTCAGGGCATTTCCTGGGAGTCTCCGTTGGATGAGGTGGATAAAATGTGCCATCTTCCAGAACCTGA
  ACTGAACAAAGAAAGACCATCTCTGCAGATTAAACTAAAAATTGAGGATTTTATCTTGCACAAAATGTTG
  GGGAAAGGAAGTTTTGGCAAGGTCTTCCTGGCAGAATTCAAGAAAACCAATCAATTTTTCGCAATAAAGG
  CCTTAAAGAAAGATGTGGTCTTGATGGACGATGATGTTGAGTGCACGATGGTAGAGAAGAGAGTTCTTTC
  CTTGGCCTGGGAGCATCCGTTTCTGACGCACATGTTTTGTACATTCCAGACCAAGGAAAACCTCTTTTTT
  GTGATGGAGTACCTCAACGGAGGGGACTTAATGTACCACATCCAAAGCTGCCACAAGTTCGACCTTTCCA
  GAGCGACGTTTTATGCTGCTGAAATCATTCTTGGTCTGCAGTTCCTTCATTCCAAAGGAATAGTCTACAG
  GGACCTGAAGCTAGATAACATCCTGTTAGACAAAGATGGACATATCAAGATCGCGGATTTTGGAATGTGC
  AAGGAGAACATGTTAGGAGATGCCAAGACGAATACCTTCTGTGGGACACCTGACTACATCGCCCCAGAGA
  TCTTGCTGGGTCAGAAATACAACCACTCTGTGGACTGGTGGTCCTTCGGGGTTCTCCTTTATGAAATGCT
  GATTGGTCAGTCGCCTTTCCACGGGCAGGATGAGGAGGAGCTCTTCCACTCCATCCGCATGGACAATCCC
  TTTTACCCACGGTGGCTGGAGAAGGAAGCAAAGGACCTTCTGGTGAAGCTCTTCGTGCGAGAACCTGAGA
  AGAGGCTGGGCGTGAGGGGAGACATCCGCCAGCACCCTTTGTTTCGGGAGATCAACTGGGAGGAACTTGA
  ACGGAAGGAGATTGACCCACCGTTCCGGCCGAAAGTGAAATCACCATTTGACTGCAGCAATTTCGACAAA
  GAATTCTTAAACGAGAAGCCCCGGCTGTCATTTGCCGACAGAGCACTGATCAACAGCATGGACCAGAATA
  TGTTCAGGAACTTTTCCTTCATGAACCCCGGGATGGAGCGGCTGATATCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282644
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282644.1, NP_001269573.1
• RefSeq Size: 3293 bp
• RefSeq ORF: 2013 bp
• Locus ID: 5588
• Cytogenetics: 10p15.1
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, T cell receptor signaling pathway, Tight junction, Vascular smooth muscle contraction
• Gene Summary: 'Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' region. This difference results in translation initiation at an alternate AUG and results in an isoform (3) with a shorter and distinct N-terminus, compared to isoform 1.