NDST1 (NM_001301063) Human Untagged Clone

CAT#: SC337535

NDST1 (untagged) - Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 (NDST1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDST1
• Synonyms: HSST; MRT46; NST1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001301063, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTGCCCTGGCATGCCTCCGGAGGCTGTGTCGGCACGTGTCCCCGCAGGCTGTCCTTTTCCTGCTGT
  TCATCTTCTGCCTGTTCAGCGTTTTCATCTCGGCCTACTACCTATATGGCTGGAAGCGAGGCCTGGAGCC
  CTCGGCGGATGCCCCCGAGCCTGACTGCGGGGACCCGCCGCCTGTGGCCCCCAGTCGCCTGCTGCCACTC
  AAGCCTGTGCAGGCAGCCACCCCTTCCCGCACAGACCCGTTGGTGCTGGTCTTTGTGGAGAGCCTCTACT
  CGCAACTGGGCCAGGAGGTGGTGGCCATCCTGGAGTCCAGCCGCTTCAAATACCGCACAGAGATTGCGCC
  GGGCAAGGGTGACATGCCCACGCTCACTGACAAGGGCCGTGGCCGCTTCGCCCTCATCATCTATGAGAAC
  ATCCTCAAGTATGTCAACCTGGACGCCTGGAACCGGGAGCTGCTGGACAAGTACTGTGTGGCCTACGGCG
  TGGGCATCATTGGCTTCTTCAAGGCCAATGAGAACAGCCTGCTGAGTGCGCAGCTCAAGGGCTTCCCCCT
  GTTCCTGCACTCAAACCTGGGCCTGAAGGACTGCAGCATCAACCCCAAGTCCCCGCTGCTCTACGTGACG
  CGACCTAGCGAGGTGGAGAAAGGTGTGCTCCCCGGCGAGGACTGGACGGTTTTCCAGTCAAATCACTCCA
  CCTATGAGCCAGTGCTGCTGGCCAAGACGCGCTCGTCTGAGTCCATCCCACACCTGGGCGCAGACGCCGG
  CCTGCATGCTGCACTGCACGCCACTGTGGTCCAGGACCTGGGCCTGCACGACGGCATCCAGCGCGTGCTG
  TTTGGCAACAACCTGAACTTCTGGCTGCACAAGCTTGTCTTCGTGGATGCCGTGGCCTTCCTCACGGGGA
  AGCGCCTCTCCCTGCCATTGGACCGCTACATCCTGGTGGACATTGATGACATCTTCGTGGGCAAGGAGGG
  CACACGCATGAAGGTGGAGGACGTGAAGGCCCTGTTTGACACACAGAACGAACTACGCGCACACATCCCA
  AACTTCACCTTCAACCTGGGCTACTCAGGGAAATTCTTCCACACAGGTACCAATGCTGAGGACGCTGGGG
  ATGATCTGCTGCTGTCGTATGTGAAGGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCCCA
  CCTTTTCCACAACCAGTCCGTGTTGGCCGAGCAGATGGCCTTGAACAAGAAGTTCGCTGTCGAGCATGGC
  ATTCCCACAGACATGGGGTATGCAGTGGCGCCCCACCACTCGGGCGTGTACCCCGTGCACGTGCAGCTGT
  ACGAGGCTTGGAAGCAGGTGTGGAGCATCCGCGTGACCAGCACGGAGGAGTACCCCCACCTGAAGCCAGC
  CCGCTACCGCCGTGGCTTCATCCACAATGGCATCATGGTTCTCCCACGGCAGACCTGCGGCCTCTTCACA
  CACACCATCTTCTACAACGAGTACCCTGGCGGCTCCAGTGAGCTGGACAAGATCATCAACGGGGGCGAGC
  TCTTCCTCACCGTGCTCCTCAATCCTATCAGCATCTTCATGACGCACCTGTCCAACTATGGGAATGACCG
  CCTGGGCCTGTACACCTTCAAGCACCTGGTGCGCTTCCTGCACTCCTGGACGAACCTCCGGCTGCAGACA
  CTGCCCCCTGTGCAGTTGGCGCAGAAGTACTTCCAGATCTTCTCCGAGGAGAAGGACCCGCTCTGGCAGG
  ACCCCTGCGAGGACAAACGTCACAAAGACATCTGGTCCAAGGAGAAGACGTGTGACCGCTTCCCAAAGCT
  CCTCATCATCGGCCCCCAGAAAACAGGCACCACTGCCCTCTACCTGTTCCTGGGCATGCACCCTGACCTA
  AGCAGCAACTACCCCAGCTCTGAGACCTTTGAGGAGATCCAGTTTTTTAATGGCCACAACTATCACAAAG
  GCATCGACTGGTACATGGAGTTCTTCCCCATCCCTTCCAACACCACCTCCGACTTCTACTTTGAGAAAAG
  CGCCAACTACTTTGATTCAGAAGTGGCGCCCCGGCGGGCAGCAGCCCTCTTGCCCAAAGCCAAGGTCCTG
  ACCATCCTCATCAACCCCGCGGACCGGGCCTATTCCTGGTACCAGATTCTGGTCTTGGATGGCAAACTGC
  TTCGCACAGAACCTGCCAAAGTGATGGACATGGTGCAGAAGTTCCTTGGGGTGACCAACACCATTGACTA
  CCACAAAACCTTGGCGTTTGATCCAAAGAAAGGATTTTGGTGCCAACTGCTTGAAGGAGGAAAAACCAAG
  TGTCTGGGCAAAAGCAAGGGCCGGAAATATCCCGAGATGGACTTGGATTCCCGAGCCTTCCTGAAGGACT
  ATTACCGGGACCACAACATCGAGCTCTCCAAGCTGCTGTATAAGATGGGCCAGACACTTCCCACTTGGCT
  ACGAGAGGACCTCCAGAACACCAGGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301063
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301063.1, NP_001287992.1
• RefSeq Size: 7970 bp
• RefSeq ORF: 2478 bp
• Locus ID: 3340
• Cytogenetics: 5q33.1
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]'
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.