DNA Ligase I (LIG1) (NM_001289064) Human Untagged Clone

CAT#: SC337573

LIG1 (untagged) - Human ligase I, DNA, ATP-dependent (LIG1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001289064, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGCGAAGTATCATGTCATTTTTCCACCCCAAGAAAGAGGGTAAAGCAAAGAAGCCTGAGAAGGAGG
  CATCCAATAGCAGCAGAGAGACGGAGCCCCCTCCAAAGGCGGCACTGAAGGAGTGGAATGGAGTGGTGTC
  CGAGAGTGACTCTCCGGTGAAGAGGCCAGGGAGGAAGGCGGCCCGGGTCCTGGGCAGCGAAGGGGAAGAG
  GAGGATGAAGCCCTTAGCCCTGCTAAAGGCCAGAAGCCTGCCCTGGACTGCTCACAGGTCTCCCCGCCCC
  GTCCTGCCACATCTCCTGAGAACAATGCTTCCCTCTCTGACACCTCTCCCATGGACAGTTCCCCATCAGG
  GATTCCGAAGCGTCGCACAGAAGCAGAGACCCCGACGGAAAGCGTTTCAGAGCCTGAGGTGGCCACGAAG
  CAGGAACTGCAGGAGGAGGAAGAGCAGACCAAGCCTCCCCGCAGAGCTCCCAAGACGCTCAGCAGCTTCT
  TCACCCCCCGGAAGCCAGCAGTCAAAAAAGAAGTGAAGGAAGAGGAGCCAGGGGCTCCAGGAAAGGAGGG
  AGCTGCTGAGGGACCCCTGGATCCATCTGGTTACAATCCTGCCAAGAACAACTATCATCCCGTGGAAGAT
  GCCTGCTGGAAACCGGGCCAGAAGGTTCCTTACCTGGCTGTGGCCCGGACGTTTGAGAAGATCGAGGAGG
  TGTCTGCTCGGCTCCGGATGGTGGAGACGCTGAGCAACTTGCTGCGCTCCGTGGTGGCCCTGTCGCCTCC
  AGACCTCCTCCCTGTCCTCTACCTCAGCCTCAACCACCTTGGGCCACCCCAGCAGGGCCTGGAGCTTGGC
  GTGGGTGATGGTGTCCTTCTCAAGGCAGTGGCCCAGGCCACAGGTCGGCAGCTGGAGTCCGTCCGGGCTG
  AGGCAGCCGAGAAAGGCGACGTGGGGCTGGTGGCCGAGAACAGCCGCAGCACCCAGAGGCTCATGCTGCC
  ACCACCTCCGCTCACTGCCTCCGGGGTCTTCAGCAAGTTCCGCGACATCGCCAGGCTCACTGGCAGTGCT
  TCCACAGCCAAGAAGATAGACATCATCAAAGGCCTCTTTGTGGCCTGCCGCCACTCAGAAGCCCGGTTCA
  TCGCTAGGTCCCTGAGCGGACGGCTGCGCCTTGGGCTGGCAGAGCAGTCGGTGCTGGCTGCCCTCTCCCA
  GGCAGTGAGCCTCACGCCCCCGGGCCAAGAATTCCCACCAGCCATGGTGGATGCTGGGAAGGGCAAGACA
  GCAGAGGCCAGAAAGACGTGGCTGGAGGAGCAAGGCATGATCCTGAAGCAGACGTTCTGCGAGGTTCCCG
  ACCTGGACCGAATTATCCCCGTGCTGCTGGAGCACGGCCTGGAACGTCTCCCGGAGCACTGCAAGCTGAG
  CCCAGGGATTCCCCTGAAACCAATGTTGGCCCATCCCACCCGGGGCATCAGCGAGGTCCTGAAACGCTTT
  GAGGAGGCAGCTTTCACCTGCGAATACAAATATGACGGGCAGAGGGCACAGATCCACGCCCTGGAAGGCG
  GGGAGGTGAAGATCTTCAGCAGGAATCAGGAAGACAACACTGGGAAGTACCCGGACATCATCAGCCGCAT
  CCCCAAGATTAAACTCCCATCGGTCACATCCTTCATCCTGGACACCGAAGCCGTGGCTTGGGACCGGGAA
  AAGAAGCAGATCCAGCCATTCCAAGTGCTCACCACCCGCAAACGCAAGGAGGTGGATGCGTCTGAGATCC
  AGGTGCAGGTGTGTTTGTACGCCTTCGACCTCATCTACCTCAATGGAGAGTCCCTGGTACGTGAGCCCCT
  TTCCCGGCGCCGGCAGCTGCTCCGGGAGAACTTTGTGGAGACAGAGGGCGAGTTTGTCTTCGCCACCTCC
  CTGGACACCAAGGACATCGAGCAGATCGCCGAGTTCCTGGAGCAGTCAGTGAAAGACTCCTGCGAGGGGC
  TGATGGTGAAGACCCTGGATGTTGATGCCACCTACGAGATCGCCAAGAGATCGCACAACTGGCTCAAGCT
  GAAGAAGGACTACCTTGATGGCGTGGGTGACACCCTGGACCTGGTGGTGATCGGCGCCTACCTGGGCCGG
  GGGAAGCGGGCCGGCCGGTACGGGGGCTTCCTGCTGGCCTCCTACGACGAGGACAGTGAGGAGCTGCAGG
  CCATATGCAAGCTTGGAACTGGCTTCAGTGATGAGGAGCTGGAGGAGCATCACCAGAGCCTCAAGGCGCT
  GGTGCTGCCCAGCCCACGCCCTTACGTGCGGATAGATGGCGCTGTGATTCCCGACCACTGGCTGGACCCC
  AGCGCTGTGTGGGAGGTGAAGTGCGCTGACCTCTCCCTCTCTCCCATCTACCCTGCTGCGCGGGGCCTGG
  TGGATAGTGACAAGGGCATCTCCCTTCGCTTCCCTCGGTTTATTCGAGTCCGTGAAGACAAGCAGCCGGA
  GCAGGCCACCACCAGTGCTCAGGTGGCCTGTTTGTACCGGAAGCAAAGTCAGATTCAGAACCAACAAGGC
  GAGGACTCAGGCTCTGACCCTGAAGATACCTACTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289064
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289064.1, NP_001275993.1
• RefSeq Size: 3206 bp
• RefSeq ORF: 2556 bp
• Locus ID: 3978
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair, DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: 'This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]'