MTR (NM_001291940) Human Untagged Clone

CAT#: SC337589

MTR (untagged) - Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTR
• Synonyms: cblG; HMAG; MS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001291940, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAGCCCAGGTGTTGGATGTCAACATGGATGATGGCATGCTAGATGGTCCAAGTGCAATGACCAGAT
  TTTGCAACTTAATTGCTTCCGAGCCAGACATCGCAAAGGTACCTTTGTGCATCGACTCCTCCAATTTTGC
  TGTGATTGAAGCTGGGTTAAAGTGCTGCCAAGGGAAGTGCATTGTCAATAGCATTAGTCTGAAGGAAGGA
  GAGGACGACTTCTTGGAGAAGGCCAGGAAGATTAAAAAGTATGGAGCTGCTATGGTGGTCATGGCTTTTG
  ATGAAGAAGGACAGGCAACAGAAACAGACACAAAAATCAGAGTGTGCACCCGGGCCTACCATCTGCTTGT
  GAAAAAACTGGGCTTTAATCCAAATGACATTATTTTTGACCCTAATATCCTAACCATTGGGACTGGAATG
  GAGGAACACAACTTGTATGCCATTAATTTTATCCATGCAACAAAAGTCATTAAAGAAACATTACCTGGAG
  CCAGAATAAGTGGAGGTCTTTCCAACTTGTCCTTCTCCTTCCGAGGAATGGAAGCCATTCGAGAAGCAAT
  GCATGGGGTTTTCCTTTACCATGCAATCAAGTCTGGCATGGACATGGGGATAGTGAATGCTGGAAACCTC
  CCTGTGTATGATGATATCCATAAGGAACTTCTGCAGCTCTGTGAAGATCTCATCTGGAATAAAGACCCTG
  AGGCCACTGAGAAGCTCTTACGTTATGCCCAGACTCAAGGCACAGGAGGGAAGAAAGTCATTCAGACTGA
  TGAGTGGAGAAATGGCCCTGTCGAAGAACGCCTTGAGTATGCCCTTGTGAAGGGCATTGAAAAACATATT
  ATTGAGGATACTGAGGAAGCCAGGTTAAACCAAAAAAAATATCCCCGACCTCTCAATATAATTGAAGGAC
  CCCTGATGAATGGAATGAAAATTGTTGGTGATCTTTTTGGAGCTGGAAAAATGTTTCTACCTCAGGTTAT
  AAAGTCAGCCCGGGTTATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATGGAAAAAGAAAGAGAAGAA
  ACCAGAGTGCTTAACGGCACAGTAGAAGAAGAGGACCCTTACCAGGGCACCATCGTGCTGGCCACTGTTA
  AAGGCGACGTGCACGACATAGGCAAGAACATAGTTGGAGTAGTCCTTGGCTGCAATAATTTCCGAGTTAT
  TGATTTAGGAGTCATGACTCCATGTGATAAGATACTGAAAGCTGCTCTTGACCACAAAGCAGATATAATT
  GGCCTGTCAGGACTCATCACTCCTTCCCTGGATGAAATGATTTTTGTTGCCAAGGAAATGGAGAGATTAG
  CTATAAGGATTCCATTGTTGATTGGAGGAGCAACCACTTCAAAAACCCACACAGCAGTTAAAATAGCTCC
  GAGATACAGTGCACCTGTAATCCATGTCCTGGACGCGTCCAAGAGTGTGGTGGTGTGTTCCCAGCTGTTA
  GATGAAAATCTAAAGGATGAATACTTTGAGGAAATCATGGAAGAATATGAAGATATTAGACAGGACCATT
  ATGAGTCTCTCAAGGAGAGGAGATACTTACCCTTAAGTCAAGCCAGAAAAAGTGGTTTCCAAATGGATTG
  GCTGTCTGAACCTCACCCAGTGAAGCCCACGTTTATTGGGACCCAGGTCTTTGAAGACTATGACCTGCAG
  AAGCTGGTGGACTACATTGACTGGAAGCCTTTCTTTGATGTCTGGCAGCTCCGGGGCAAGTACCCGAATC
  GAGGCTTTCCCAAGATATTTAACGACAAAACAGTAGGTGGAGAGGCCAGGAAGGTCTACGATGATGCCCA
  CAATATGCTGAACACACTGATTAGTCAAAAGAAACTCCGGGCCCGGGGTGTGGTTGGGTTCTGGCCAGCA
  CAGAGTATCCAAGACGACATTCACCTGTACGCAGAGGCTGCTGTGCCCCAGGCTGCAGAGCCCATAGCCA
  CCTTCTATGGGTTAAGGCAACAGGCTGAGAAGGACTCTGCCAGCACGGAGCCATACTACTGCCTCTCAGA
  CTTCATCGCTCCCTTGCATTCTGGCATCCGTGACTACCTGGGCCTGTTTGCCGTTGCCTGCTTTGGGGTA
  GAAGAGCTGAGCAAGGCCTATGAGGATGATGGTGACGACTACAGCAGCATCATGGTCAAGGCGCTGGGGG
  ACCGGCTGGCAGAGGCCTTTGCAGAAGAGCTCCATGAAAGAGTTCGCCGAGAACTGTGGGCCTACTGTGG
  CAGTGAGCAGCTGGACGTCGCAGACCTGCGCAGGCTGCGGTACAAGGGCATCCGCCCGGCTCCTGGCTAC
  CCCAGCCAGCCCGACCACACCGAGAAGCTCACCATGTGGAGACTCGCAGACATCGAGCAGTCTACAGGCA
  TTAGGTTAACAGAATCATTAGCAATGGCACCTGCTTCAGCAGTCTCAGGCCTCTACTTCTCCAATTTGAA
  GTCCAAATATTTTGCTGTGGGGAAGATTTCCAAGGATCAGGTTGAGGATTATGCATTGAGGAAGAACATA
  TCTGTGGCTGAGGTTGAGAAATGGCTTGGACCCATTTTGGGATATGATACAGACTAA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001291940
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291940.1, NP_001278869.1
• RefSeq Size: 10462 bp
• RefSeq ORF: 2577 bp
• Locus ID: 4548
• Cytogenetics: 1q43
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, One carbon pool by folate
• Gene Summary: 'This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (3) lacks an internal exon, which causes translation initiation at a downstream AUG, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.