RPB2 (POLR2B) (NM_001303268) Human Untagged Clone

CAT#: SC337856

POLR2B (untagged) - Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR2B
• Synonyms: hRPB140; POL2RB; RPB2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001303268, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGGATTGTAATCAGCCACGATATTTGCTGAAGTTTGAACAAATTTATCTTTCCAAGCCTACCCATT
  GGGAAAGAGATGGTGCTCCTTCACCAATGATGCCCAATGAAGCTAGATTAAGGAATCTCACGTATTCTGC
  TCCGCTTTATGTTGATATAACAAAAACAGTCATTAAAGAAGGTGAAGAACAACTTCAGACTCAGCATCAG
  AAAACTTTTATAGGAAAAATTCCAATTATGTTGCGGTCAACTTACTGCCTTTTGAATGGCTTGACAGATC
  GTGATCTTTGTGAGTTAAATGAATGCCCTTTGGATCCTGGTGGCTATTTCATTATTAATGGATCAGAAAA
  GGTTCTGATTGCCCAAGAGAAAATGGCAACAAACACAGTTTATGTGTTTGCCAAAAAGGATTCTAAATAT
  GCCTACACAGGAGAGTGTAGATCATGTCTTGAGAATTCTTCCCGACCCACCAGTACTATATGGGTTAGCA
  TGCTGGCAAGAGGAGGACAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGTGGCAACTCTACCATA
  TATCAAGCAAGAAGTTCCCATCATTATTGTGTTCAGAGCATTAGGTTTTGTGTCCGACAGAGATATTTTA
  GAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGTTAAACCTTCTCTCGATGAAGCTT
  TTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCAAGAGGAGCAAAGCCTGGTGTTACTAA
  AGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCATGTTGGTGTCAGTGAT
  TTTTGTGAGACCAAAAAAGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTCTGGCAGCTTTGGGTA
  GAAGAGAACTAGATGACAGAGATCACTATGGAAACAAGAGATTGGATCTTGCTGGGCCGCTGCTTGCATT
  CTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAGAAATTTATTGATCGA
  GGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCCTAAAATACTCTTTAG
  CTACTGGAAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATCTCAGGTGTTAAACCG
  CCTGACTTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAATTCTCCTATTGGTAGAGACGGCAAGCTA
  GCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCCTGCCGAGACCCCAGAGGGCCATG
  CTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCTCAACCATCTCCAATTCT
  GGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCAGCTATTGCTGATGCAACC
  AAGATTTTTGTTAATGGCTGCTGGGTTGGAATACATAAAGATCCCGAACAACTTATGAACACCCTAAGGA
  AATTGAGACGTCAGATGGACATCATTGTGTCTGAAGTTTCTATGATCAGAGATATTCGAGAGAGGGAGAT
  TCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTGGAAAAACAAAAGCTACTTTTG
  AAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTATAGTTGGCAGGATCTTGTGGCCA
  GTGGGGTAGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATGCTTGCAATGACTCCAGATGATTT
  ACAGGAGAAAGAAGTAGCTTATTGTTCCACATATACACACTGTGAGATTCATCCCTCAATGATCCTTGGT
  GTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCTAGAAACACATACCAGTCTGCTATGG
  GTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGCATGGACACATTGGCCCATGTTCTCTA
  TTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAATATCTACGATTTAGAGAGCTGCCAGCAGGC
  ATCAACTCAATTGTGGCCATTGCATCATACACTGGATATAATCAGGAAGACTCTGTTATCATGAATCGTT
  CAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTATCGCTCATACAAAGAACAGGAGTCTAAAAAAGG
  ATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAAACATGCCAGGGCATGAGGCATGCCATTTAC
  GACAAGCTGGATGATGATGGTTTGATAGCTCCAGGGGTTCGTGTATCAGGAGATGATGTTATTATAGGCA
  AAACAGTCACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGACGCTATACCAAGAGAGACTG
  TAGCACTTTTCTCAGAACTAGCGAGACGGGCATTGTGGATCAGGTTATGGTAACTCTCAATCAGGAAGGA
  TATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGGATTCCACAGATTGGAGACAAATTTGCTAGTCGAC
  ATGGTCAAAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGATATGCCTTTCACCTGTGAAGGTATCAC
  CCCTGATATCATCATCAATCCCCATGCCATCCCCTCTCGTATGACTATTGGTCACTTAATTGAATGCCTT
  CAAGGGAAGGTATCGGCTAACAAGGGTGAAATTGGTGATGCCACTCCATTTAATGATGCTGTTAACGTGC
  AGAAGATTTCTAATCTTTTATCTGATTATGGCTATCATCTCAGAGGAAATGAGGTCCTGTACAATGGGTT
  CACTGGTCGAAAAATCACATCACAAATATTTATTGGCCCCACTTATTACCAGCGTTTGAAGCATATGGTG
  GATGATAAGATTCACTCTCGTGCTAGGGGACCTATTCAGATCCTCAATAGACAGCCCATGGAGGGTAGAT
  CTCGTGATGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTGCCCATGGAGCAGCCCAGTT
  TTTAAGGGAAAGATTGTTTGAGGCATCAGATCCATATCAGGTTCATGTTTGCAATCTTTGTGGAATAATG
  GCGATTGCCAACACCAGGACCCATACATATGAATGCAGGGGCTGCCGCAATAAAACCCAGATTTCTTTGG
  TGCGAATGCCTTACGCATGCAAACTATTGTTTCAGGAACTTATGTCTATGAGTATTGCACCGCGAATGAT
  GAGTGTTTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001303268
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303268.1, NP_001290197.1
• RefSeq Size: 3911 bp
• RefSeq ORF: 3300 bp
• Locus ID: 5431
• Cytogenetics: 4q12
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: 'This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]'
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks an alternate exon in the 5' coding region, and initiates translation at an alternate downstream start codon, compared to variant 1. This results in isoform 2, which is shorter and has a distinct N-terminus, compared to isoform 1.