NEDD4 (NM_001284338) Human Untagged Clone

CAT#: SC338000

NEDD4 (untagged) - Human neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 3


  "NM_001284338" in other vectors (1)

Reconstitution Protocol

USD 1,350.00

5 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NEDD4
Synonyms NEDD4-1; RPF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001284338, the custom clone sequence may differ by one or more nucleotides


ATGGCACAAAGCTTACGATTGCACTTTGCAGCCAGAAGAAGCAATACTTACCCTTTGTCAGAAACCTCCG
GAGATGACTTGGATAGCCATGTTCACATGTGCTTCAAAAGACCAACACGGATTTCAACGTCTAACGTTGT
TCAAATGAAGCTGACTCCCAGACAGACTGCACTAGCTCCGTTAATAAAGGAAAACGTTCAGTCTCAAGAA
AGATCATCTGTTCCCTCATCTGAAAATGTTAATAAAAAGAGCAGCTGTCTACAGATTTCACTACAGCCAA
CAAGGTACAGTGGATATCTTCAGTCTAGCAATGTCTTAGCTGATAGTGATGATGCTTCGTTTACTTGTAT
CTTGAAGGATGGTATTTACAGTAGTGCTGTGGTCGATAATGAATTGAATGCTGTGAATGATGGTCACCTT
GTAAGCAGTCCAGCCATTTGTAGTGGTAGCCTTAGTAACTTTTCAACCAGTGATAATGGGTCTTACAGCA
GCAACGGTAGTGATTTTGGGTCATGTGCAAGTATCACAAGTGGAGGTTCATATACTAACAGTGTCATCAG
TGACAGTAGTAGTTATACTTTTCCACCAAGTGATGATACTTTTTTGGGTGGAAACTTACCTTCTGACAGC
ACCTCCAATAGAAGTGTGCCAAACAGGAATACTACTCCTTGTGAAATTTTTTCAAGAAGTACAAGTACAG
ATCCTTTTGTCCAGGATGACTTGGAACATGGATTAGAGATTATGAAATTGCCAGTGAGCAGGAACACAAA
AATTCCACTAAAACGTTACTCCTCCTTAGTCATTTTTCCTAGGAGTCCTTCAACTACCCGACCGACTTCT
CCAACAAGTCTGTGTACTCTTCTGAGCAAAGGATCCTATCAAACTTCACACCAGTTTATTATTTCTCCTA
GTGAAATTGCACATAATGAGGATGGCACTAGTGCTAAAGGATTTCTTTCAACAGCTGTCAATGGACTTCG
GTTATCTAAAACAATTTGTACCCCCGGAGAAGTAAGAGACATACGGCCGCTTCACAGGAAGGGCTCGTTA
CAGAAGAAAATTGTTCTTTCGAATAATACTCCCAGACAGACTGTCTGTGAAAAGTCATCTGAAGGATATT
CTTGTGTTTCAGTGCATTTCACCCAACGAAAAGCAGCTACATTAGACTGTGAAACAACAAATGGTGATTG
TAAACCAGAAATGTCAGAAATTAAGCTTAATTCTGATTCAGAGTATATTAAGCTCATGCATAGGACATCT
GCATGTTTGCCATCCTCCCAAAATGTAGATTGTCAAATAAATATCAATGGAGAATTGGAAAGACCACATT
CACAGATGAACAAAAACCATGGTATTTTACGAAGAAGTATTTCATTGGGAGGAGCTTATCCAAATATTTC
CTGTCTATCCAGCCTTAAGCACAATTGTTCTAAAGGGGGACCATCTCAGTTACTCATAAAGTTTGCATCT
GGAAATGAAGGTAAAGTGGATAATTTATCAAGAGACAGCAACAGAGATTGCACAAATGAACTGTCTAATT
CTTGCAAGACAAGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGACAGAAAATCC
AAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTCATCCAAGAAGTCACAAATCAAGAGTTAAA
GGTTATCTGAGACTAAAAATGACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGG
CTGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTTGCCATTTGCAGCAACAACA
AGAACCTTCTCCTCTACCTCCAGGGTGGGAAGAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAAC
CATGAATCTAGAAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTGAGAATGGCA
ACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGGCAGATATCCGAGGAAACAGAAAGTGTTGA
CAACCGAGAGTCTTCCGAGAACTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAACCAGGCC
TTCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCAGAAGAATTGAATGCCAGAC
TCACCATTTTTGGAAATTCAGCCGTGAGCCAGCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAG
CTTACAAGCCTATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCTGGATTACCA
CCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATATTATGTAGATCACAATTCCAGAACGACTA
CTTGGACAAAGCCCACTGTACAGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGG
CCCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCCATCTGAAATTGAGCAAGGA
TTCCTTCCTAAAGGCTGGGAAGTCCGGCATGCACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTA
AAACCACCACCTGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATCACTTGATAC
TTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGAGAACTCACACAGATGGAAGAATCTTCTAC
ATAAATCACAATATAAAAAGAACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAG
CAGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAAAGTTGAAGAAGCAGAATGA
CATTCCAAACAAATTTGAAATGAAACTTCGCCGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATG
GGTGTCAAGAGAGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGGGATTGGATT
ATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAGGAAATGTTTAACCCTTATTATGGGTTGTT
TGAATATTCTGCTACGGACAATTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCAC
CTCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCATGGCAAACTGTTGGATGGTT
TTTTCATCCGCCCATTTTACAAGATGATGCTTCACAAACCAATAACCCTTCATGATATGGAATCTGTGGA
TAGTGAATATTACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTCAGGTTTATC
ATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCTGAAAAATGGTGGATCAGAAATAGTTGTCA
CCAATAAGAACAAAAAGGAATATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCA
AATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCATCAAAATTTTTGATGAAAAT
GAACTAGAGCTTCTTATGTGTGGACTGGGAGATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATA
AAAATGGCTACAGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGATGGATTCAGA
AAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTCGGGTGCCTATGAATGGATTTGCTGAACTA
TACGGTTCAAATGGACCACAGTCATTTACAGTTGAACAGTGGGGTACTCCTGAAAAGCTGCCAAGAGCTC
ATACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAATTATGGGATAAACTTCAGAT
GGCAATTGAAAACACCCAGGGCTTTGATGGAGTTGATTAG


Restriction Sites SgfI-MluI     
ACCN NM_001284338
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001284338.1, NP_001271267.1
RefSeq Size 7240 bp
RefSeq ORF 3960 bp
Locus ID 4734
Cytogenetics 15q21.3
Protein Families Druggable Genome
Protein Pathways Endocytosis, Ubiquitin mediated proteolysis
Gene Summary 'This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]'
Transcript Variant: This variant (3) contains three alternate in-frame exons, compared to variant 2. The encoded isoform (3) is longer than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.