TJP1 (NM_001301026) Human Untagged Clone

CAT#: SC338094

TJP1 (untagged) - Human tight junction protein 1 (TJP1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TJP1
• Synonyms: ZO-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001301026, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGAGAAATACGTTTTCAATGGATTGCCACAGTAAGAGCACAGCAATGGAGGAAACAGCTATATGGG
  AACAACATACAGTGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATTGCAATATCTGGTGGACGAGA
  TAATCCTCATTTTCAGAGTGGGGAAACGTCAATAGTGATTTCAGATGTGCTGAAAGGAGGACCAGCTGAA
  GGACAGCTACAGGAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAATGTTGAACATGCTT
  TTGCTGTTCAGCAACTAAGGAAAAGTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCA
  AATACCAGTAAGTCGTCCTGATCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATA
  CATGATCCAAGAAGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGGATAGAA
  GTGCAAGTAGAGAGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTCCAGCCAGCCTGCTAA
  ACCTACTAAAGTCACACTGGTGAAATCCCGGAAAAATGAAGAATATGGTCTTCGATTGGCAAGCCATATA
  TTTGTTAAGGAAATTTCACAAGATAGTTTGGCAGCAAGAGATGGCAATATTCAAGAAGGTGATGTTGTAT
  TGAAGATAAATGGTACTGTGACAGAAAATATGTCATTGACAGATGCAAAGACATTGATAGAAAGGTCTAA
  AGGCAAATTAAAAATGGTAGTTCAAAGAGATGAACGGGCTACGCTATTGAATGTCCCTGATCTTTCTGAC
  AGCATCCACTCTGCTAATGCCTCTGAGAGAGACGACATTTCAGAAATTCAGTCACTGGCATCAGATCATT
  CTGGTCGATCACACGATAGGCCTCCCCGCCGCAGCCGGTCACGATCTCCTGACCAGCGGTCAGAGCCTTC
  TGATCATTCCAGGCACTCGCCGCAGCAGCCAAGCAATGGCAGTCTCCGGAGTAGAGATGAAGAGAGAATT
  TCTAAACCTGGGGCTGTCTCAACTCCTGTAAAGCATGCTGATGATCACACACCTAAAACAGTGGAAGAAG
  TTACAGTTGAAAGAAATGAGAAACAAACACCTTCTCTTCCAGAACCAAAGCCTGTGTATGCCCAAGTTGG
  GCAACCAGATGTGGATTTACCTGTCAGTCCATCTGATGGTGTCCTACCTAATTCAACTCATGAAGATGGG
  ATTCTTCGGCCCAGCATGAAATTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGGCTGGCTGGTG
  GAAATGATGTTGGAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAGGAAGGCTTAGAGGA
  AGGTGATCAAATTCTCAGGGTAAACAACGTAGATTTTACAAATATCATAAGAGAAGAAGCCGTCCTTTTC
  CTGCTTGACCTCCCTAAAGGAGAAGAAGTGACCATATTGGCTCAGAAGAAGAAGGATGTTTATCGTCGCA
  TTGTAGAATCAGATGTAGGAGATTCTTTCTATATTAGAACCCATTTTGAATATGAAAAGGAATCTCCCTA
  TGGACTTAGTTTTAACAAAGGAGAGGTGTTCCGTGTTGTGGATACCTTGTACAATGGAAAACTGGGCTCT
  TGGCTTGCTATTCGAATTGGTAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAG
  CTGAGCAGCTAGCCAGTGTACAGTATACACTTCCAAAAACAGCAGGCGGAGACCGTGCTGACTTCTGGAG
  ATTCAGAGGTCTTCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGATTTGTCCGCTCAGCCTGTT
  CAAACAAAGTTTCCAGCTTATGAAAGAGTGGTTCTTCGAGAAGCTGGATTTCTGAGGCCTGTAACCATTT
  TTGGACCAATAGCTGATGTTGCCAGAGAAAAGCTGGCAAGAGAAGAACCAGATATTTATCAAATTGCAAA
  GAGTGAACCACGAGACGCTGGAACTGACCAACGTAGCTCTGGCATTATTCGCCTGCATACAATAAAGCAA
  ATCATAGATCAAGACAAACATGCTTTATTAGATGTAACACCAAATGCAGTTGATCGTCTTAACTATGCCC
  AGTGGTATCCAATTGTTGTATTTCTTAACCCTGATTCTAAGCAAGGAGTAAAAACAATGAGAATGAGGTT
  ATGTCCAGAATCTCGGAAAAGTGCCAGGAAGTTATACGAGCGATCTCATAAACTTCGTAAAAATAATCAC
  CATCTTTTTACAACTACAATTAACTTAAATTCAATGAATGATGGTTGGTATGGTGCGCTGAAAGAAGCAA
  TTCAACAACAGCAAAACCAGCTGGTATGGGTTTCCGAGGGAAAGGCGGATGGTGCTACAAGTGATGACCT
  TGATTTGCATGATGATCGTCTGTCCTACCTGTCAGCTCCAGGTAGTGAATACTCAATGTATAGCACGGAC
  AGTAGACACACTTCTGACTATGAAGACACAGACACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATG
  AAACTCTTAATGATGAGGTTGGGACTCCACCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGA
  GGACTCCTCTGGAATGCATCATGAAAACCAAACATATCCTCCTTACTCACCACAAGCGCAGCCACAACCA
  ATTCATAGAATAGACTCCCCTGGATTTAAGCCAGCCTCTCAACAGGTGTATAGAAAGGATCCATATCCCG
  AGGAAATGATGAGGCAGAACCATGTTTTGAAACAGCCAGCCGTTAGTCACCCAGGGCACAGGCCAGACAA
  AGAGCCTAATCTGACCTATGAACCCCAACTCCCATACGTAGAGAAACAAGCCAGCAGAGACCTCGAGCAG
  CCCACATACAGATACGAGTCCTCAAGCTATACGGACCAGTTTTCTCGAAACTATGAACATCGTCTGCGAT
  ACGAAGATCGCGTCCCCATGTATGAAGAACAGTGGTCATATTATGATGACAAACAGCCCTACCCATCTCG
  GCCACCTTTTGATAATCAGCACTCTCAAGACCTTGACTCCAGACAGCATCCCGAAGAGTCCTCAGAACGA
  GGGTACTTTCCACGTTTTGAAGAGCCAGCCCCTCTGTCTTACGACAGCAGACCACGTTACGAACAGGCAC
  CTAGAGCATCCGCCCTGCGGCACGAAGAGCAGCCAGCTCCTGGGTATGACACACATGGTAGACTCAGACC
  GGAAGCCCAGCCCCACCCTTCAGCAGGGCCCAAGCCTGCAGAGTCCAAGCAGTATTTTGAGCAATATTCA
  CGCAGTTACGAGCAAGTACCACCCCAAGGATTTACCTCTAGAGCAGGTCATTTTGAGCCTCTCCATGGTG
  CTGCAGCTGTCCCTCCGCTGATACCTTCATCTCAGCATAAGCCAGAAGCTCTGCCTTCAAACACCAAACC
  ACTGCCTCCACCCCCAACTCAAACCGAAGAAGAGGAAGATCCAGCAATGAAGCCACAGTCTGTACTCACC
  AGAGTTAAGATGTTTGAAAACAAAAGATCTGCATCCTTAGAGACCAAGAAGGATGTAAATGACACTGGCA
  GTTTTAAGCCTCCAGAAGTAGCATCTAAACCTTCAGGTGCTCCCATCATTGGTCCCAAACCCACTTCTCA
  GAATCAATTCAGTGAACATGACAAAACTCTGTACAGGATCCCAGAACCTCAAAAACCTCAACTGAAGCCA
  CCTGAAGATATTGTTCGGTCCAATCATTATGACCCTGAAGAAGATGAAGAATATTATCGAAAACAGCTGT
  CATACTTTGACCGAAGAAGTTTTGAGAATAAGCCTCCTGCACACATTGCCGCCAGCCATCTCTCCGAGCC
  TGCAAAGCCAGCGCATTCTCAGAATCAATCAAATTTTTCTAGTTATTCTTCAAAGGGAAAGCCTCCTGAA
  GCTGATGGTGTGGATAGATCATTTGGCGAGAAACGCTATGAACCCATCCAGGCCACTCCCCCTCCTCCTC
  CATTGCCCTCGCAGTATGCCCAGCCATCTCAGCCTGTCACCAGCGCGTCTCTCCACATACATTCTAAGGG
  AGCACATGGTGAAGGTAATTCAGTGTCATTGGATTTTCAGAATTCCTTAGTGTCCAAACCAGACCCACCT
  CCATCTCAGAATAAGCCAGCAACTTTCAGACCACCAAACCGAGAAGATACTGCTCAGGCAGCTTTCTATC
  CCCAGAAAAGTTTTCCAGATAAAGCCCCAGTTAATGGAACTGAACAGACTCAGAAAACAGTCACTCCAGC
  ATACAATCGATTCACACCAAAACCATATACAAGTTCTGCCCGACCATTTGAACGCAAGTTTGAAAGTCCT
  AAATTCAATCACAATCTTCTGCCAAGTGAAACTGCACATAAACCTGACTTGTCTTCAAAAACTCCCACTT
  CTCCAAAAACTCTTGTGAAATCGCACAGTTTGGCACAGCCTCCTGAGTTTGACAGTGGAGTTGAAACTTT
  CTCTATCCATGCAGAGAAGCCTAAATATCAAATAAATAATATCAGCACAGTGCCTAAAGCTATTCCTGTG
  AGTCCTTCAGCTGTGGAAGAGGATGAAGATGAAGATGGTCATACTGTGGTGGCCACAGCCCGAGGCATAT
  TTAACAGCAATGGGGGCGTGCTGAGTTCCATAGAAACTGGTGTTAGTATAATTATCCCTCAAGGAGCCAT
  TCCCGAAGGAGTTGAGCAGGAAATCTATTTCAAGGTCTGCCGGGACAACAGCATCCTTCCACCTTTAGAT
  AAAGAGAAAGGTGAAACACTGCTGAGTCCTTTGGTGATGTGTGGTCCCCATGGCCTCAAGTTCCTGAAGC
  CTGTGGAGCTGCGCTTACCACACTGTGCGTCCATGACTCCTGACGGTTGGTCTTTTGCTCTAAAATCATC
  CGACTCCTCGTCGGGTGATCCTAAAACCTGGCAAAACAAGTGTCTTCCCGGAGATCCAAATTATCTCGTT
  GGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301026
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301026.1, NP_001287955.1
• RefSeq Size: 7570 bp
• RefSeq ORF: 5079 bp
• Locus ID: 7082
• Cytogenetics: 15q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection
• Gene Summary: 'This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (4) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it lacks an alternate in-frame exon in the central coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.