TJP1 (NM_001301026) Human Untagged Clone

CAT#: SC338094

TJP1 (untagged) - Human tight junction protein 1 (TJP1), transcript variant 4


  "NM_001301026" in other vectors (1)

Reconstitution Protocol

USD 1,720.00

6 Weeks*

Size
    • 10 ug

Product Images

Other products for "TJP1"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TJP1
Synonyms ZO-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001301026, the custom clone sequence may differ by one or more nucleotides


ATGGAGAGAAATACGTTTTCAATGGATTGCCACAGTAAGAGCACAGCAATGGAGGAAACAGCTATATGGG
AACAACATACAGTGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATTGCAATATCTGGTGGACGAGA
TAATCCTCATTTTCAGAGTGGGGAAACGTCAATAGTGATTTCAGATGTGCTGAAAGGAGGACCAGCTGAA
GGACAGCTACAGGAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAATGTTGAACATGCTT
TTGCTGTTCAGCAACTAAGGAAAAGTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCA
AATACCAGTAAGTCGTCCTGATCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATA
CATGATCCAAGAAGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGGATAGAA
GTGCAAGTAGAGAGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTCCAGCCAGCCTGCTAA
ACCTACTAAAGTCACACTGGTGAAATCCCGGAAAAATGAAGAATATGGTCTTCGATTGGCAAGCCATATA
TTTGTTAAGGAAATTTCACAAGATAGTTTGGCAGCAAGAGATGGCAATATTCAAGAAGGTGATGTTGTAT
TGAAGATAAATGGTACTGTGACAGAAAATATGTCATTGACAGATGCAAAGACATTGATAGAAAGGTCTAA
AGGCAAATTAAAAATGGTAGTTCAAAGAGATGAACGGGCTACGCTATTGAATGTCCCTGATCTTTCTGAC
AGCATCCACTCTGCTAATGCCTCTGAGAGAGACGACATTTCAGAAATTCAGTCACTGGCATCAGATCATT
CTGGTCGATCACACGATAGGCCTCCCCGCCGCAGCCGGTCACGATCTCCTGACCAGCGGTCAGAGCCTTC
TGATCATTCCAGGCACTCGCCGCAGCAGCCAAGCAATGGCAGTCTCCGGAGTAGAGATGAAGAGAGAATT
TCTAAACCTGGGGCTGTCTCAACTCCTGTAAAGCATGCTGATGATCACACACCTAAAACAGTGGAAGAAG
TTACAGTTGAAAGAAATGAGAAACAAACACCTTCTCTTCCAGAACCAAAGCCTGTGTATGCCCAAGTTGG
GCAACCAGATGTGGATTTACCTGTCAGTCCATCTGATGGTGTCCTACCTAATTCAACTCATGAAGATGGG
ATTCTTCGGCCCAGCATGAAATTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGGCTGGCTGGTG
GAAATGATGTTGGAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAGGAAGGCTTAGAGGA
AGGTGATCAAATTCTCAGGGTAAACAACGTAGATTTTACAAATATCATAAGAGAAGAAGCCGTCCTTTTC
CTGCTTGACCTCCCTAAAGGAGAAGAAGTGACCATATTGGCTCAGAAGAAGAAGGATGTTTATCGTCGCA
TTGTAGAATCAGATGTAGGAGATTCTTTCTATATTAGAACCCATTTTGAATATGAAAAGGAATCTCCCTA
TGGACTTAGTTTTAACAAAGGAGAGGTGTTCCGTGTTGTGGATACCTTGTACAATGGAAAACTGGGCTCT
TGGCTTGCTATTCGAATTGGTAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAG
CTGAGCAGCTAGCCAGTGTACAGTATACACTTCCAAAAACAGCAGGCGGAGACCGTGCTGACTTCTGGAG
ATTCAGAGGTCTTCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGATTTGTCCGCTCAGCCTGTT
CAAACAAAGTTTCCAGCTTATGAAAGAGTGGTTCTTCGAGAAGCTGGATTTCTGAGGCCTGTAACCATTT
TTGGACCAATAGCTGATGTTGCCAGAGAAAAGCTGGCAAGAGAAGAACCAGATATTTATCAAATTGCAAA
GAGTGAACCACGAGACGCTGGAACTGACCAACGTAGCTCTGGCATTATTCGCCTGCATACAATAAAGCAA
ATCATAGATCAAGACAAACATGCTTTATTAGATGTAACACCAAATGCAGTTGATCGTCTTAACTATGCCC
AGTGGTATCCAATTGTTGTATTTCTTAACCCTGATTCTAAGCAAGGAGTAAAAACAATGAGAATGAGGTT
ATGTCCAGAATCTCGGAAAAGTGCCAGGAAGTTATACGAGCGATCTCATAAACTTCGTAAAAATAATCAC
CATCTTTTTACAACTACAATTAACTTAAATTCAATGAATGATGGTTGGTATGGTGCGCTGAAAGAAGCAA
TTCAACAACAGCAAAACCAGCTGGTATGGGTTTCCGAGGGAAAGGCGGATGGTGCTACAAGTGATGACCT
TGATTTGCATGATGATCGTCTGTCCTACCTGTCAGCTCCAGGTAGTGAATACTCAATGTATAGCACGGAC
AGTAGACACACTTCTGACTATGAAGACACAGACACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATG
AAACTCTTAATGATGAGGTTGGGACTCCACCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGA
GGACTCCTCTGGAATGCATCATGAAAACCAAACATATCCTCCTTACTCACCACAAGCGCAGCCACAACCA
ATTCATAGAATAGACTCCCCTGGATTTAAGCCAGCCTCTCAACAGGTGTATAGAAAGGATCCATATCCCG
AGGAAATGATGAGGCAGAACCATGTTTTGAAACAGCCAGCCGTTAGTCACCCAGGGCACAGGCCAGACAA
AGAGCCTAATCTGACCTATGAACCCCAACTCCCATACGTAGAGAAACAAGCCAGCAGAGACCTCGAGCAG
CCCACATACAGATACGAGTCCTCAAGCTATACGGACCAGTTTTCTCGAAACTATGAACATCGTCTGCGAT
ACGAAGATCGCGTCCCCATGTATGAAGAACAGTGGTCATATTATGATGACAAACAGCCCTACCCATCTCG
GCCACCTTTTGATAATCAGCACTCTCAAGACCTTGACTCCAGACAGCATCCCGAAGAGTCCTCAGAACGA
GGGTACTTTCCACGTTTTGAAGAGCCAGCCCCTCTGTCTTACGACAGCAGACCACGTTACGAACAGGCAC
CTAGAGCATCCGCCCTGCGGCACGAAGAGCAGCCAGCTCCTGGGTATGACACACATGGTAGACTCAGACC
GGAAGCCCAGCCCCACCCTTCAGCAGGGCCCAAGCCTGCAGAGTCCAAGCAGTATTTTGAGCAATATTCA
CGCAGTTACGAGCAAGTACCACCCCAAGGATTTACCTCTAGAGCAGGTCATTTTGAGCCTCTCCATGGTG
CTGCAGCTGTCCCTCCGCTGATACCTTCATCTCAGCATAAGCCAGAAGCTCTGCCTTCAAACACCAAACC
ACTGCCTCCACCCCCAACTCAAACCGAAGAAGAGGAAGATCCAGCAATGAAGCCACAGTCTGTACTCACC
AGAGTTAAGATGTTTGAAAACAAAAGATCTGCATCCTTAGAGACCAAGAAGGATGTAAATGACACTGGCA
GTTTTAAGCCTCCAGAAGTAGCATCTAAACCTTCAGGTGCTCCCATCATTGGTCCCAAACCCACTTCTCA
GAATCAATTCAGTGAACATGACAAAACTCTGTACAGGATCCCAGAACCTCAAAAACCTCAACTGAAGCCA
CCTGAAGATATTGTTCGGTCCAATCATTATGACCCTGAAGAAGATGAAGAATATTATCGAAAACAGCTGT
CATACTTTGACCGAAGAAGTTTTGAGAATAAGCCTCCTGCACACATTGCCGCCAGCCATCTCTCCGAGCC
TGCAAAGCCAGCGCATTCTCAGAATCAATCAAATTTTTCTAGTTATTCTTCAAAGGGAAAGCCTCCTGAA
GCTGATGGTGTGGATAGATCATTTGGCGAGAAACGCTATGAACCCATCCAGGCCACTCCCCCTCCTCCTC
CATTGCCCTCGCAGTATGCCCAGCCATCTCAGCCTGTCACCAGCGCGTCTCTCCACATACATTCTAAGGG
AGCACATGGTGAAGGTAATTCAGTGTCATTGGATTTTCAGAATTCCTTAGTGTCCAAACCAGACCCACCT
CCATCTCAGAATAAGCCAGCAACTTTCAGACCACCAAACCGAGAAGATACTGCTCAGGCAGCTTTCTATC
CCCAGAAAAGTTTTCCAGATAAAGCCCCAGTTAATGGAACTGAACAGACTCAGAAAACAGTCACTCCAGC
ATACAATCGATTCACACCAAAACCATATACAAGTTCTGCCCGACCATTTGAACGCAAGTTTGAAAGTCCT
AAATTCAATCACAATCTTCTGCCAAGTGAAACTGCACATAAACCTGACTTGTCTTCAAAAACTCCCACTT
CTCCAAAAACTCTTGTGAAATCGCACAGTTTGGCACAGCCTCCTGAGTTTGACAGTGGAGTTGAAACTTT
CTCTATCCATGCAGAGAAGCCTAAATATCAAATAAATAATATCAGCACAGTGCCTAAAGCTATTCCTGTG
AGTCCTTCAGCTGTGGAAGAGGATGAAGATGAAGATGGTCATACTGTGGTGGCCACAGCCCGAGGCATAT
TTAACAGCAATGGGGGCGTGCTGAGTTCCATAGAAACTGGTGTTAGTATAATTATCCCTCAAGGAGCCAT
TCCCGAAGGAGTTGAGCAGGAAATCTATTTCAAGGTCTGCCGGGACAACAGCATCCTTCCACCTTTAGAT
AAAGAGAAAGGTGAAACACTGCTGAGTCCTTTGGTGATGTGTGGTCCCCATGGCCTCAAGTTCCTGAAGC
CTGTGGAGCTGCGCTTACCACACTGTGCGTCCATGACTCCTGACGGTTGGTCTTTTGCTCTAAAATCATC
CGACTCCTCGTCGGGTGATCCTAAAACCTGGCAAAACAAGTGTCTTCCCGGAGATCCAAATTATCTCGTT
GGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTTTAA


Restriction Sites SgfI-MluI     
ACCN NM_001301026
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001301026.1, NP_001287955.1
RefSeq Size 7570 bp
RefSeq ORF 5079 bp
Locus ID 7082
Cytogenetics 15q13.1
Protein Families Druggable Genome
Protein Pathways Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection
Gene Summary 'This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]'
Transcript Variant: This variant (4) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it lacks an alternate in-frame exon in the central coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.