E1 (NC_008188) Virus Tagged ORF Clone

CAT#: VC101840

Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Human papillomavirus type 103], codon optimized for human cell expression, YP_656494

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101840 represents NCBI reference of YP_656494 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATAAGGATACTAAAGGGACATCCTGCTTTACCGACATTACCGCCAACGCTTGCGAATTTATTG
  AAACTGAGGCTACCTGTGAGGACGGCCTGGACTCCCTGGAGGCTCTGTTTGAGGAATCTGATGGTACTGA
  TATCAGCGAGCTGATAGAGAACATTGATGAGCCTGATCGCGGCAATCCTCAAGCACTCTTGAACCGCCAG
  CAGCTGGAGGAAGACAGTCAGTTGCTGACCGTTCTCAAGCGCAAGTACGTTTCCCCTTCTCCGAAACAAC
  GGGTGATAGATCTGTCACCTCGACTGGAAGCTCTGTGTGTGTCTCCGAGAGGATCCTCCTCAAAACGGCG
  CCTGTTCGAAGACAGCGGTCTCGGACATGAGCCTGAAGATTCCAATGCCGAAGTTGAAAAAGTGGCCTCT
  GAGGCTCTCACCAACAGCGGGACTCTGACTAATGGCGATGAGAACGCTGTGTGTGCTACTCAGGACGGAG
  ACACACGCTGCCTGGACATCCTTCATTCTTCCAATCGCCAAGCGACGGCACTTACCAAATTCAAGAATGT
  CTTTGGGGTAGGGTACAAAGAAATTACGCGACCATTCCAATCCAACAAAAGCTGCTGTCACTCCTGGGTA
  GCAGCGATCTTCGGAGTAGTGGCTGAGATGCTCGAAGCCGCAAAGACCCTGTTCAAGGTTCATTGCGATT
  ATCTTCAGATTATCAACCCAAGTATGGGCACAGGCGTCACCGTGCTGTGCTTGTTTGAGTTCTCCAGTAG
  TAAGTGTCGCGATACAGTGCAGAAGCTTATCTCTAATGTGCTCAACGTGCAGGAACATCAGATCATTACC
  GACCCCCCCAGGCACCGGAGCGTGCCCGTTGCGCTGTTTTTCTATAAGCAGAGCATGTCCAATACCAGCT
  TTGTGCACGGAACCATGCCTGACTGGCTGAAACGGCAAACCATGCTGAACCACCAGCAAGAGGCCGACAG
  CTTTGAGTTGTCTCATATGGTGCAGTGGGCCTACGACAACAATATTTGGGACGAGGCAGAGCTGGCCTAT
  CAGTACGCCTGTCTCGCTGATGTGGAGCCCAACGCCGCCGCCTGGCTTAAATCTAACCAACAATACAAGT
  ACGTCAGTGATTGCGCCAAGATGGTGCGAATGTATAAAAAGTATGAAATGCAGCAAATGTCCATGGCACA
  GTGGATTAAAAAGTGTGGAGAGCACATTGAGACAGACGGCGATTGGAAGAAGATCATCAACCTGCTTAAG
  TACCAAGAGATTTCAGTGATTGCCTTTCTGACCAGTTTCCGCATGTTCCTGAAAGGGCAGCCAAAAAAGA
  ACTGCATAGCCCTGTGGGGACCACCTGATACCGGAAAAAGTATGTTCTGCTATAGCCTGATCAGGTACGT
  GAAAGGCAAAGTCGTTTCTTACGCTAACAGCAAATCCCAATTCTGGCTGCAGCCCTTGACCGATGCTAAA
  CTCGGTCTCATAGATGACGCCACTTTTCCATGTTTTCAATTTATGGACGTCTACATGCGATCTGCACTTG
  ATGGGAACGAAGTTTCTGTGGACTGTAAACACCGGGTACCTGTGCAAATTAAGCTGCCGCCACTGCTGGT
  GACAAGTAATATTGACATGCATAGTGAGGCCTCCCTGAAATACCTTCAAAGCCGGATTACCAGCTTTAAG
  TTCCCACACAAACTCCCGCTGGATACTAACGGGAATCCCATCTTCATTATCACCGATACTGACTGGAAGT
  GCTTCTTCTCCAAGCTCGAACAGCAGCTTGATCTTTGCGATCCAGAAGATAGTACGAACGGAGACCCAGG
  AGGAGCCTTCAGATGTTGCCCCAGGGAGACAACAGGCGCTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101840 representing YP_656494
  Red=Cloning sites Green=Tags
  
  MEDKDTKGTSCFTDITANACEFIETEATCEDGLDSLEALFEESDGTDISELIENIDEPDRGNPQALLNRQ
  QLEEDSQLLTVLKRKYVSPSPKQRVIDLSPRLEALCVSPRGSSSKRRLFEDSGLGHEPEDSNAEVEKVAS
  EALTNSGTLTNGDENAVCATQDGDTRCLDILHSSNRQATALTKFKNVFGVGYKEITRPFQSNKSCCHSWV
  AAIFGVVAEMLEAAKTLFKVHCDYLQIINPSMGTGVTVLCLFEFSSSKCRDTVQKLISNVLNVQEHQIIT
  DPPRHRSVPVALFFYKQSMSNTSFVHGTMPDWLKRQTMLNHQQEADSFELSHMVQWAYDNNIWDEAELAY
  QYACLADVEPNAAAWLKSNQQYKYVSDCAKMVRMYKKYEMQQMSMAQWIKKCGEHIETDGDWKKIINLLK
  YQEISVIAFLTSFRMFLKGQPKKNCIALWGPPDTGKSMFCYSLIRYVKGKVVSYANSKSQFWLQPLTDAK
  LGLIDDATFPCFQFMDVYMRSALDGNEVSVDCKHRVPVQIKLPPLLVTSNIDMHSEASLKYLQSRITSFK
  FPHKLPLDTNGNPIFIITDTDWKCFFSKLEQQLDLCDPEDSTNGDPGGAFRCCPRETTGAL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_008188
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_008188.1, YP_656494
• RefSeq ORF: 1863
• Locus ID: 5177014
• MW: 70.1 kDa