E2 (NC_001583) Virus Tagged ORF Clone

CAT#: VC101910

Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 26], codon optimized for human cell expression, NP_041785

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101910 represents NCBI reference of NP_041785 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAATCTCTGCCAGCGACTCAACGCCTGCCAGGAGAAGATTCTCGACTACTATGAGCTGGATAGCA
  ACAAACTGACGGACCAGATCGACTATTGGAAGCTGGTGCGCTACGAATGCGCTATTTTTTATAAAGCCCG
  CGAGGGAAACATGCAGTGTATTAACCACCAAGTGGTCCCTTCTACTGTTGTCTGCAAACAAAAGGCTTGG
  CAAGCTATCGAGATCCATATCGCTCTGCAGTCCTTGATTAACACCGACTACAATACCGAGGCTTGGACCA
  TGCGAGATACCAGTTACGAAATGTACATGACTGAACCTAAGCACTGTTTCAAAAAAGAAGGAACCACAGT
  GACAGTGGTGTTTGATTGCAACAAGGAAAACACAATGGATTACATTCGCTGGAAGTACGTCTATTATAAG
  ACCGACATTGGCTGGTGTAAAGGCACCGGAGACGTTGACGCCAAAGGAATATATTATACGCAGGGCGCAT
  ATAAACAGTATTATGTGGACTTCAAGCAAGAGGCCGAGAAGTATGGGACCGGAGTGCAATGGGCAGTGCA
  CGTGTGCGGGCAGGTCATTTGCTGCCCGGAATTTGTTAGTTCAACTTGTTCCAGTAATCAGATCTCTACA
  GCGAAGACCGCGGAACCCGTCTCAAATGCCACCACCCAGACCACAGAGGCCTACGTACCTGTAGGCACCA
  AAGAAACAGAAGCCCCTTATCCAGGGAAACGGCGCCGCCTGTCCGGCCCTGATACAACCGTTACAACAGT
  TACTACAGTGACAACTGCCGCCACTCAGCCAGGGCAGTCAGTCGATTATACCAATAACAACCTGCACTCT
  ACGAGTGGGGGACACCATCCAGGCCGGGATACAAGCTCTGATCAGACAGTGTTCATCGTACACTTGAAGG
  GCGATACCAATTCTCTGAAATGCCTCAGATACCGGTTCAAGAAACATAAAGGACTCTACTGTAACGTTAG
  TTCTACGTGGCATTGGACAAGTAATGACACTAATCAGCAGGGTATCGTAACTATAACTTTTAACTCTATC
  ACACAGCGGAACAATTTTCTCACAACCGTGAAAATTCCACAGTCAATTACTTCAACCCTTGGCATCATGT
  CCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101910 representing NP_041785
  Red=Cloning sites Green=Tags
  
  MENLCQRLNACQEKILDYYELDSNKLTDQIDYWKLVRYECAIFYKAREGNMQCINHQVVPSTVVCKQKAW
  QAIEIHIALQSLINTDYNTEAWTMRDTSYEMYMTEPKHCFKKEGTTVTVVFDCNKENTMDYIRWKYVYYK
  TDIGWCKGTGDVDAKGIYYTQGAYKQYYVDFKQEAEKYGTGVQWAVHVCGQVICCPEFVSSTCSSNQIST
  AKTAEPVSNATTQTTEAYVPVGTKETEAPYPGKRRRLSGPDTTVTTVTTVTTAATQPGQSVDYTNNNLHS
  TSGGHHPGRDTSSDQTVFIVHLKGDTNSLKCLRYRFKKHKGLYCNVSSTWHWTSNDTNQQGIVTITFNSI
  TQRNNFLTTVKIPQSITSTLGIMSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001583
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001583.1, NP_041785
• RefSeq ORF: 1125
• Locus ID: 1496945
• MW: 42.6 kDa