E7 (NC_001586) Virus Tagged ORF Clone

CAT#: VC101914

Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 32], codon optimized for human cell expression, NP_041802

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101914 represents NCBI reference of NP_041802 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGCAACGCCCCCACACTGAAAGATATTATCCTCTATGACTTGCCAACTTGCGACCCTACAACAT
  GCGATACACCACCCGTTGACTTGTACTGCTATGAACAGTTTGACACTAGTGACGAAGATGATGAGGATGA
  CGATCAGCCGATAAAGCAGGACATACAGAGGTATCGGATAGTCTGTGGTTGCACTCAGTGCGGAAGATCC
  GTCAAGCTGGTCGTTTCCAGTACTGGCGCAGATATTCAGCAGCTGCATCAGATGCTTTTGGATACCCTGG
  GTATCGTCTGCCCTTTGTGCGCCTGTGTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101914 representing NP_041802
  Red=Cloning sites Green=Tags
  
  MRGNAPTLKDIILYDLPTCDPTTCDTPPVDLYCYEQFDTSDEDDEDDDQPIKQDIQRYRIVCGCTQCGRS
  VKLVVSSTGADIQQLHQMLLDTLGIVCPLCACVE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001586
• ORF Size: 312 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001586.1, NP_041802
• RefSeq ORF: 312
• Locus ID: 1489425
• MW: 11.6 kDa