E6 (NC_001693) Virus Tagged ORF Clone

CAT#: VC101978

Myc-DDK-tagged ORF clone of viral ORF for transforming protein E6 [Human papillomavirus type 60], codon optimized for human cell expression, NP_043437

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101978 represents NCBI reference of NP_043437 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATGGAGGAAGATAGATTTCCAACAACAGTTGCCGATTATTGCAGTGAGTTCGACATCCCACTGA
  AGGATCTCAAGCTCAAATGCGTATTTTGTCGATTCTACCTGACTGAACAACAGTTGGCTGCCTTTTACAT
  CAAAAACCTGAAGCTGGTGTGGAAGAACCGGTACTGTTTTGCCTGTTGTACGCCCTGTCTCCGGCTCACT
  GCAAAGTTCGAGGCAGAGAATTATTTTCAATGCATGTGCAAGGGGGAGGTATTGGAGGTGCTCACTCGGA
  TTCCATTGTCCTCTCTCTCCGTCAGATGCTTTGACTGTCTGACACTCCTCTCTTTCGCAGAGAAGATCGA
  CTGTATAATTTCCGGGCAGAACTTCTACCTTGTCAGAGGGCGCTGGCGCTCTTACTGTCGGAACTGCATC
  GAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101978 representing NP_043437
  Red=Cloning sites Green=Tags
  
  MQMEEDRFPTTVADYCSEFDIPLKDLKLKCVFCRFYLTEQQLAAFYIKNLKLVWKNRYCFACCTPCLRLT
  AKFEAENYFQCMCKGEVLEVLTRIPLSSLSVRCFDCLTLLSFAEKIDCIISGQNFYLVRGRWRSYCRNCI
  EK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001693
• ORF Size: 426 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001693.1, NP_043437
• RefSeq ORF: 426
• Locus ID: 1403638
• MW: 16.8 kDa