E7 (NC_001595) Virus Tagged ORF Clone

CAT#: VC102002

Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 7], codon optimized for human cell expression, NP_041855

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102002 represents NCBI reference of NP_041855 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGGTGAGCGCCCTACCCTCGGGGACATCGTGCTGGACCTGCAACCAGAGCCAGTGAGCCTCTCAT
  GTAACGAACAATTGGACTCAAGTGATTCTGAGGATGACCATGAGCAGGATCAATTGGACAGCTCCCATAA
  TCGCCAGAGAGAACAGCCTACCCAGCAGGACCTGCAAGTGAATCTGCAGAGTTTTAAGATCGTTACTCAT
  TGTGTATTCTGCCATTGCCTCGTCCGGCTGGTAGTGCATTGCACAGCTACCGACATCCGGCAAGTGCATC
  AGCTTCTGATGGGAACTCTGAATATAGTGTGTCCCAACTGTGCCGCGACCGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102002 representing NP_041855
  Red=Cloning sites Green=Tags
  
  MHGERPTLGDIVLDLQPEPVSLSCNEQLDSSDSEDDHEQDQLDSSHNRQREQPTQQDLQVNLQSFKIVTH
  CVFCHCLVRLVVHCTATDIRQVHQLLMGTLNIVCPNCAATA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001595
• ORF Size: 333 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001595.1, NP_041855
• RefSeq ORF: 333
• Locus ID: 1489473
• MW: 12.5 kDa