E7 (NC_001596) Virus Tagged ORF Clone

CAT#: VC102016

Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041862

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102016 represents NCBI reference of NP_041862 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGGCAAAGAGGCTACCATCCCTGAGGTCGTTCTTGAGTTGCAGGAACTCGTCCAACCAACTGCCG
  ATCTCCACTGTTACGAAGAACTGACCGAGGAACCGGCAGAAGAGGAACAGTGTTTGACACCGTATAAAAT
  CGTGGCCGGGTGTGGGTGCGGCGCTCGGTTGAGACTGTACGTACTTGCTACTAATCTGGGAATTAGGGCA
  CAACAAGAGCTGCTCCTCGGCGACATACAACTGGTGTGCCCAGAATGCCGCGGCCGGCTGCGGCATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102016 representing NP_041862
  Red=Cloning sites Green=Tags
  
  MIGKEATIPEVVLELQELVQPTADLHCYEELTEEPAEEEQCLTPYKIVAGCGCGARLRLYVLATNLGIRA
  QQELLLGDIQLVCPECRGRLRHE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001596
• ORF Size: 279 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001596.1, NP_041862
• RefSeq ORF: 279
• Locus ID: 1489481
• MW: 10.4 kDa