NP (NC_003443) Virus Tagged ORF Clone

CAT#: VC102052

Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid protein [Human parainfluenza virus 2], codon optimized for human cell expression, NP_598401

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102052 represents NCBI reference of NP_598401 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAGTGTGCTGAAGACCTTTGAAAGATTCACGATTCAGCAGGAACTGCAGGAGCAGTCTGACGATA
  CGCCAGTCCCTCTCGAAACCATCAAGCCTACCATCCGCGTTTTTGTGATCAATAACAACGATCCTGTTGT
  ACGCTCACGCCTCCTGTTTTTTAACTTGCGCATCATTATGTCAAATACTGCACGCGAGGGGCACCGCGCT
  GGCGCCCTGTTGTCTCTGTTGTCCCTGCCCTCCGCAGCAATGTCCAACCACATCAAACTCGCCATGCATT
  CACCTGAAGCGTCCATCGACCGAGTGGAGATCACCGGATTCGAAAACAACTCCTTTCGGGTTATACCAGA
  CGCCAGATCCACCATGTCTCGAGGTGAGGTTCTTGCTTTCGAGGCTCTGGCCGAGGATATACCCGATACC
  TTGAATCATCAGACTCCATTCGTCAATAACGACGTGGAGGACGATATCTTCGACGAGACTGAGAAGTTCC
  TTGATGTCTGTTACTCCGTCCTGATGCAGGCCTGGATAGTGACTTGTAAATGTATGACAGCCCCAGATCA
  GCCGCCCGTGAGCGTCGCAAAGATGGCTAAGTACCAACAGCAGGGTAGGATCAATGCTAGGTATGTTCTG
  CAACCTGAGGCACAGAGACTGATTCAAAATGCAATTAGAAAATCTATGGTGGTCCGCCATTTTATGACAT
  ATGAACTGCAACTGAGTCAGAGCCGGTCCCTGCTGGCCAATCGCTACTACGCCATGGTGGGAGACATCGG
  CAAATACATCGAGCACTCTGGGATGGGAGGCTTCTTCCTGACGCTGAAGTATGGCCTGGGTACACGATGG
  CCTACTCTGGCTCTTGCCGCTTTTTCCGGCGAATTGCAGAAACTGAAAGCCTTGATGCTGCATTACCAAA
  GTCTTGGCCCTATGGCCAAGTACATGGCTCTCCTGGAATCACCCAAGCTCATGGATTTCGTTCCTAGTGA
  ATATCCTCTGGATTACTCATACGCAATGGGAATCGGCACTGTGCTGGACACCAATATGCGCAACTACGCA
  TACGGCCGGTCTTACCTGAATCAGCAGTATTTTCAGCTGGGCGTGGAGACAGCCCGGAAGCAGCAGGGGG
  CTGTAGACAACAGAACTGCCGAAGATCTCGGGATGACGGCTGCTGACAAAGCAGATCTGACCGCTACTAT
  CTCCAAGCTTTCTTTGAGTCAATTGCCCAGAGGTAGGCAGCCAATAAGCGATCCATTCGCAGGCGCAAAC
  GACCGAGAGATGGGAGGACAAGCAAACGACACTCCTGTATACAACTTCAACCCTATCGACACAAGGCGCT
  ACGACAACTATGACAGTGACGGAGAGGACAGAATAGACAATGATCAGGATCAGGCAATACGAGAAAATAG
  AGGCGAGCCCGGACAGCCCAATAATCAGACGAGTGACAATCAGCAGCGCTTTAACCCACCTATACCACAA
  CGCACCTCAGGAATGAGTTCAGAAGAATTCCAGCATAGTATGAACCAGTATATCCGCGCCATGCATGAGC
  AGTACCGGGGCTCTCAAGACGACGATGCTAACGATGCCACAGACGGTAATGATATTAGTCTGGAACTCGT
  CGGTGATTTCGACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102052 representing NP_598401
  Red=Cloning sites Green=Tags
  
  MSSVLKTFERFTIQQELQEQSDDTPVPLETIKPTIRVFVINNNDPVVRSRLLFFNLRIIMSNTAREGHRA
  GALLSLLSLPSAAMSNHIKLAMHSPEASIDRVEITGFENNSFRVIPDARSTMSRGEVLAFEALAEDIPDT
  LNHQTPFVNNDVEDDIFDETEKFLDVCYSVLMQAWIVTCKCMTAPDQPPVSVAKMAKYQQQGRINARYVL
  QPEAQRLIQNAIRKSMVVRHFMTYELQLSQSRSLLANRYYAMVGDIGKYIEHSGMGGFFLTLKYGLGTRW
  PTLALAAFSGELQKLKALMLHYQSLGPMAKYMALLESPKLMDFVPSEYPLDYSYAMGIGTVLDTNMRNYA
  YGRSYLNQQYFQLGVETARKQQGAVDNRTAEDLGMTAADKADLTATISKLSLSQLPRGRQPISDPFAGAN
  DREMGGQANDTPVYNFNPIDTRRYDNYDSDGEDRIDNDQDQAIRENRGEPGQPNNQTSDNQQRFNPPIPQ
  RTSGMSSEEFQHSMNQYIRAMHEQYRGSQDDDANDATDGNDISLELVGDFDS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003443
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003443.1, NP_598401
• RefSeq ORF: 1626
• Locus ID: 935191
• MW: 61.1 kDa