env (NC_011800) Virus Tagged ORF Clone

CAT#: VC102162

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein [Human T-lymphotropic virus 4], codon optimized for human cell expression, YP_002455789

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: env
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102162 represents NCBI reference of YP_002455789 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAATGTGTTGTTTCTCACACTCCTCGCAACACTTGGCATCCCCGTGCTGCAGGCTAGCAGATGTA
  CGATCACCGTGGGAATTAGCTCCTACCATTCTTCACCATGTAGCCCTGCACAGCCGCTCTGCACTTGGGC
  ACTGGACCTCGTTTCAATTACTAAAGACCAGCTCCTCTATCCCCCATGTCAGAATTTGATTACATATTCA
  AATTACCATAAAACCTATTCCTTGTATCTCTTCCCCCACTGGGTGCAAAAGCCACTGAGAAGAGGTCTCG
  GCTATTATAGCGCATCCTATTCCGACCCTTGCTCTCTGCAATGTCCCTACCTGGGTAGTCAAAGCTGGAC
  CTGTCCTTATACAGGCCCTGTGTCCAGTCCTACCTGGAGGTTTTCAACCGATGTGAATTTCACACAAGAA
  GTGAGCCGCGTCTCCCTTAAGCTCCATTTCAGTAAATGCGGGAGCTCACTTACCCTTTTGATCGACGCAC
  CAGGGTACGACCCACTGTGGTACCTGACATCAGAACCCACCCAGGAACCACCCACACCGCCACCTTTGGT
  TTCTGACAGTGACCTCGAGCATGTATTGACTCCCTCTGCATCCTGGGCTTCTAAAATGCTCACCCTGATT
  CATCTTACTCTGCAGAGTACTAATTACAGCTGCATGGTCTGTATCGATCGGGCATCTCTCTCCAGCTGGC
  ACGTGCTCTATACACCTAACATTTCCTCTAACGCTCCTAGCAAACCAATTGTTAGACCATCCCTGGCACT
  CAGCGCTCCTCGCCCGCAACCCTTTCCCTGGACGCACTGCTACCAGCCACAGGTGCAAGCCGTCACCACC
  GCAAAATGTAACAATAGCATCATACTTCCCCCATTCAGTCTCTCCCCTCTTCCAGGCGCTCCGCTGACAC
  GCCGCCGGAGAGCTGTGCCCGTAGCTGTATGGCTTGTGAGCGCACTCGCAGCAGGCACCGGAATCGCCGG
  CGGTGTTACAGGATCCCTTTCCCTGGCCAGTTCCAGAAGCCTTCTGTCAGAAGTGGACAAGGACATCAGT
  CACTTGACGAGGGCAATTGTGAAGAACCACCAGAATATCCTCAGGGTCGCTCAGTACGCGGCCCAGAATC
  GCCGGGGACTGGACCTTCTGTTTTGGGAACAGGGAGGCCTGTGTAAAGCAATCCAAGAGCAGTGCTGTTT
  TCTGAACATTTCAAACACTCACATAAGCGTGCTGCAAGAGAGACCACCCCTTGAAACACGGGTGACTACG
  GGCTGGGGCCTGAACTGGGACTTGGGTCTTAGCCAATGGGCAAGGGAAGCCTTGCAGACCGGGATTACTC
  TGTTGGCCCTGTTGCTGCTGATCATCATCCTTGGTCCGTGCATTATAAGGCAGCTCCAGGCCCTGCCCCA
  GAGACTGCAGCAGAGACCGGATCAGTATCCCCTTCTTAACCCAGAAACACCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102162 representing YP_002455789
  Red=Cloning sites Green=Tags
  
  MGNVLFLTLLATLGIPVLQASRCTITVGISSYHSSPCSPAQPLCTWALDLVSITKDQLLYPPCQNLITYS
  NYHKTYSLYLFPHWVQKPLRRGLGYYSASYSDPCSLQCPYLGSQSWTCPYTGPVSSPTWRFSTDVNFTQE
  VSRVSLKLHFSKCGSSLTLLIDAPGYDPLWYLTSEPTQEPPTPPPLVSDSDLEHVLTPSASWASKMLTLI
  HLTLQSTNYSCMVCIDRASLSSWHVLYTPNISSNAPSKPIVRPSLALSAPRPQPFPWTHCYQPQVQAVTT
  AKCNNSIILPPFSLSPLPGAPLTRRRRAVPVAVWLVSALAAGTGIAGGVTGSLSLASSRSLLSEVDKDIS
  HLTRAIVKNHQNILRVAQYAAQNRRGLDLLFWEQGGLCKAIQEQCCFLNISNTHISVLQERPPLETRVTT
  GWGLNWDLGLSQWAREALQTGITLLALLLLIIILGPCIIRQLQALPQRLQQRPDQYPLLNPETPL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011800
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011800.1, YP_002455789
• RefSeq ORF: 1455
• Locus ID: 7205459
• MW: 53.5 kDa