E1 (NC_009824) Virus Tagged ORF Clone

CAT#: VC102202

Myc-DDK-tagged ORF clone of viral ORF for E1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491550

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102202 represents NCBI reference of YP_001491550 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGAATGGCGGAACACCAGCGGCTTGTACGTACTCACTAATGATTGCTCTAACTCAAGCATTGTGT
  ATGAGGCTGACGACGTTATCTTGCACACTCCCGGTTGCGTGCCTTGTGTCCAGGATGGGAACACCTCTAC
  ATGCTGGACTCCCGTGACCCCTACGGTCGCCGTGCGCTATGTTGGCGCTACTACAGCATCAATCCGAAGT
  CACGTGGATCTCTTGGTTGGAGCAGCTACTATGTGTTCCGCGTTGTACGTTGGGGACATGTGTGGGGCCG
  TCTTTCTTGTGGGTCAGGCCTTCACCTTCCGGCCCAGAAGACATCAAACCGTGCAAACCTGCAATTGCTC
  CCTTTATCCAGGCCACCTGTCCGGGCATAGAATGGCATGGGATATGATGATGAACTGGAGTCCCGCTGTC
  GGAATGGTCGTTGCGCATGTTCTGCGCCTGCCACAGACACTGTTCGACATCATGGCCGGAGCCCACTGGG
  GAATTCTCGCAGGTCTGGCATACTACTCAATGCAGGGCAATTGGGCCAAGGTTGCCATCATAATGGTCAT
  GTTTTCCGGAGTCGATGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102202 representing YP_001491550
  Red=Cloning sites Green=Tags
  
  MLEWRNTSGLYVLTNDCSNSSIVYEADDVILHTPGCVPCVQDGNTSTCWTPVTPTVAVRYVGATTASIRS
  HVDLLVGAATMCSALYVGDMCGAVFLVGQAFTFRPRRHQTVQTCNCSLYPGHLSGHRMAWDMMMNWSPAV
  GMVVAHVLRLPQTLFDIMAGAHWGILAGLAYYSMQGNWAKVAIIMVMFSGVDA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009824
• ORF Size: 579 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009824.1, YP_001491550
• RefSeq ORF: 579
• MW: 21.0 kDa