NP (NC_007360) Virus Tagged ORF Clone

CAT#: VC102421

Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid protein [Influenza A virus (A/Goose/Guangdong/1/96(H5N1))], codon optimized for human cell expression, YP_308667

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102421 represents NCBI reference of YP_308667 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCCAGGGCACCAAGAGAAGCTACGAACAGATGGAAACCGGCGGGGAGAGACAGAATGCTACCG
  AGATCAGAGCTAGCGTCGGCCGGATGGTGGGAGGCATCGGCCGGTTCTACATCCAGATGTGCACCGAACT
  GAAGCTGTCCGACTACGAGGGCAGACTGATCCAAAACAGCATCACAATTGAGAGAATGGTGCTCAGCGCC
  TTCGACGAGCGGCGGAACAAGTACCTGGAGGAGCACCCCTCCGCCGGCAAGGACCCCAAGAAAACCGGCG
  GCCCCATTTACCGGCGGCGGGACGGCAAGTGGGTGCGGGAGCTCATTCTGTACGACAAGGAGGAGATTCG
  GCGGATCTGGCGGCAGGCCAACAACGGAGAGGACGCCACCGCCGGGCTCACCCACATGATGATCTGGCAC
  AGCAACCTGAACGACGCCACCTACCAACGGACCCGGGCACTGGTCCGGACCGGCATGGACCCCCGGATGT
  GCAGCCTGATGCAGGGCAGCACCCTGCCCCGGCGGAGCGGCGCCGCCGGGGCCGCCGTCAAGGGCGTCGG
  GACAATGGTGATGGAGCTGATTCGGATGATCAAGCGGGGCATCAACGACCGGAACTTCTGGCGGGGCGAG
  AACGGGCGGCGGACCCGGATCGCCTACGAGCGGATGTGCAACATCCTGAAGGGCAAGTTCCAGACCGCCG
  CCCAGCGGGCTATGATGGATCAGGTGCGGGAAAGCCGGAACCCCGGCAACGCCGAGATCGAGGACCTGAT
  CTTCCTCGCCCGGAGCGCCCTGATCCTCCGGGGCAGCGTGGCCCACAAGTCCTGCCTGCCTGCCTGCGTC
  TACGGCCTGGCCGTCGCAAGCGGGTACGACTTCGAACGGGAGGGCTACAGCCTGGTCGGCATCGACCCCT
  TCCGGCTGCTCCAGAACAGCCAGGTGTTCAGCCTCATCCGGCCAAACGAGAACCCTGCCCACAAGAGCCA
  GCTGGTGTGGATGGCATGCCACAGCGCAGCCTTCGAGGACCTGCGGGTGAGCAGCTTCATCCGGGGCACC
  CGGGTCGCCCCCCGGGGGCAGCTGTCCACCAGAGGCGTGCAGATCGCCTCCAACGAGAACATGGAGACCA
  TGGACAGCAGCACCCTGGAGCTGCGGTCCCGGTACTGGGCCATCAGAACCCGGTCCGGCGGCAACACCAA
  CCAGCAGCGGGCCAGCGCAGGGCAAATCTCCGTGCAGCCCACCTTCAGCGTGCAGCGGAACCTGCCTTTC
  GAGCGGGCCACCATCATGGCCGCCTTCACCGGCAATACCGAAGGCCGGACCAGCGACATGCGGACCGAGA
  TTATCCGGATGATGGAGTCCAGCCGGCCCGAGGACGTGTCCTTCCAGGGCCGGGGCGTGTTTGAGCTGAG
  CGACGAGAAGGCCACCAACCCCATCGTGCCCAGCTTCGACATGTCCAACGAGGGGTCCTACTTCTTCGGC
  GACAACGCAGAGGAATACGACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007360
• ORF Size: 1494 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007360.1, YP_308667
• RefSeq ORF: 1494
• Locus ID: 3654618
• MW: 56.3 kDa