PB1-F2 (NC_004911) Virus Tagged ORF Clone

CAT#: VC102441

Myc-DDK-tagged ORF clone of viral ORF for PB1-F2 proein [Influenza A virus (A/Hong Kong/1073/99(H9N2))], codon optimized for human cell expression, YP_473349

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PB1-F2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102441 represents NCBI reference of YP_473349 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAGGAGCAGGACACACCATGGACCCAGAGTACAGAACACATTAACATTCAGAAAAAAGGGGGCG
  GACAGCAAACTCAGCGCCCCGAACATCCCAATTCAACGCTGCTGATGGACCACTACCTGAAAACTATGTC
  ACGCGCAGGCATGCACAAGCAGATAGTCTATTGGAAGCAGTGGCTGAGTCTTAAGAACCCAACGCAGGAC
  TCCCTCAAGACCCATGTATTGAAAAGGTGGAAACTGTCTTCTAAACGCGAGTGGATCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102441 representing YP_473349
  Red=Cloning sites Green=Tags
  
  MEQEQDTPWTQSTEHINIQKKGGGQQTQRPEHPNSTLLMDHYLKTMSRAGMHKQIVYWKQWLSLKNPTQD
  SLKTHVLKRWKLSSKREWIS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004911
• ORF Size: 270 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004911.1, YP_473349
• RefSeq ORF: 270
• Locus ID: 3896426
• MW: 10.8 kDa