M2 (NC_007377) Virus Tagged ORF Clone

CAT#: VC102450

Myc-DDK-tagged ORF clone of viral ORF for membrane protein M2 [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308853

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: M2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102450 represents NCBI reference of YP_308853 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCTGCTCACTGAAGTCGAGACGCCAATACGGAACGAATGGGGATGCAGGTGCAATGACTCCAGCG
  ATCCTCTGGTGGTGGCCGCTTCTATTATTGGCATTCTGCACTTCATTCTTTGGATCTTGGACAGACTCTT
  CTTTAAATGTATATATCGCTTTTTTAAACACGGATTGAAGCGCGGTCCATCAACCGAGGGTGTGCCAGAG
  AGCATGAGGGAGGAGTACCGCAAGGAGCAGCAAAGCGCCGTAGATGCCGATGACTCCCATTTTGTGTCAA
  TCGAGTTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102450 representing YP_308853
  Red=Cloning sites Green=Tags
  
  MSLLTEVETPIRNEWGCRCNDSSDPLVVAASIIGILHFILWILDRLFFKCIYRFFKHGLKRGPSTEGVPE
  SMREEYRKEQQSAVDADDSHFVSIELE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007377
• ORF Size: 291 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007377.1, YP_308853
• RefSeq ORF: 291
• Locus ID: 3655107
• MW: 11.2 kDa