NP (NC_007381) Virus Tagged ORF Clone

CAT#: VC102454

Myc-DDK-tagged ORF clone of viral ORF for nucleoprotein [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308871

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102454 represents NCBI reference of YP_308871 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTCAGGGAACAAAACGGAGTTACGAGCAAATGGAGACCGATGGAGAGCGACAGAATGCCACAG
  AAATCAGAGCTTCTGTCGGCAAGATGATTGATGGGATAGGGCGGTTCTACATCCAGATGTGCACAGAGCT
  CAAACTTTCTGACTATGAGGGGAGGTTGATCCAAAACTCACTGACCATCGAACGAATGGTGCTGTCTGCT
  TTCGACGAGAGAAGAAACAAATACCTCGAAGAACACCCTTCAGCCGGCAAGGATCCCAAGAAAACAGGTG
  GCCCCATATACAAGAGGGTCGACGGCAAGTGGATGCGGGAGCTGGTGCTTTACGACAAGGAGGAGATCAG
  GCGGATATGGCGGCAGGCCAATAATGGGGATGATGCGACAGCCGGGCTGACCCACATGATGATATGGCAC
  AGCAATCTCAACGATACAACCTATCAACGCACACGAGCCCTTGTGCGGACAGGAATGGATCCAAGGATGT
  GTAGCCTGATGCAGGGTAGTACGCTGCCCAGACGCAGTGGCGCCGCTGGCGCTGCAGTAAAGGGAGTCGG
  CACCATGGTCATGGAGCTGATCCGGATGATCAAAAGGGGCATAAACGACCGGAATTTTTGGCGGGGCGAA
  AATGGACGCAAAACGCGGAGCGCTTACGAGAGAATGTGTAACATCCTGAAAGGCAAGTTCCAGACTGCTG
  CTCAACGCGCTATGATGGATCAGGTCCGGGAGAGCCGGAACCCCGGGAACGCCGAGATTGAAGATTTGAT
  ATTCCTGGCCCGCAGTGCGCTGATTTTGCGCGGATCAGTTGCTCATAAGAGCTGCCTTCCCGCGTGCGTT
  TATGGCCCAGCTATTGCAAGCGGATATAACTTCGAGAAAGAGGGATATTCCCTCGTAGGAATTGATCCAT
  TTAAACTTCTGCAGAATAGTCAAGTGTACTCTCTCATTAGGCCAAACGAAAACCCCGCTCATAAGTCCCA
  GTTGGTATGGATGGCCTGTAACAGCGCCGCCTTTGAAGACTTGAGAGTGCTTTCCTTCATCCGCGGCACA
  AAGGTGAGCCCACGAGGCAAGCTGTCAACAAGAGGTGTCCAGATTGCCTCAAATGAAAATATGGATACAA
  TGGAAAGCTCCACTTTGGAACTCAGGAGCCGCTATTGGGCCATCCGCACCCGCTCAGGCGGCAACACTAA
  CCAGCAGCGGGCCTCCGCTGGCCAGATTTCTGTACAGCCTGCGTTCAGTGTTCAGAGAAACCTGCCATTC
  GACAAGCCCACAATTATGGCAGCCTTCACTGGGAATACAGAAGGTCGGACTTCTGATATGCGAGCAGAGA
  TTATCCGCATGATGGAAGGCGCAAAACCCGAAGAAATGAGTTTTCAGGGACGCGGAGTGTTTGAGTTGAG
  CGACGAGAAGGCTACCAATCCCATCGTCCCATCCTTTGACATGAGTAATGAGGGTTCCTATTTTTTTGGC
  GACAACGCCGAGGAGTATGACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102454 representing YP_308871
  Red=Cloning sites Green=Tags
  
  MASQGTKRSYEQMETDGERQNATEIRASVGKMIDGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSA
  FDERRNKYLEEHPSAGKDPKKTGGPIYKRVDGKWMRELVLYDKEEIRRIWRQANNGDDATAGLTHMMIWH
  SNLNDTTYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELIRMIKRGINDRNFWRGE
  NGRKTRSAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACV
  YGPAIASGYNFEKEGYSLVGIDPFKLLQNSQVYSLIRPNENPAHKSQLVWMACNSAAFEDLRVLSFIRGT
  KVSPRGKLSTRGVQIASNENMDTMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISVQPAFSVQRNLPF
  DKPTIMAAFTGNTEGRTSDMRAEIIRMMEGAKPEEMSFQGRGVFELSDEKATNPIVPSFDMSNEGSYFFG
  DNAEEYDN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007381
• ORF Size: 1494 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007381.1, YP_308871
• RefSeq ORF: 1494
• Locus ID: 3655111
• MW: 56.1 kDa