NP (NC_002019) Virus Tagged ORF Clone

CAT#: VC102478

Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid protein [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, NP_040982

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102478 represents NCBI reference of NP_040982 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCCAGGGCACCAAACGGTCATACGAGCAGATGGAAACCGACGGCGAAAGGCAGAATGCGACCG
  AGATTAGGGCTTCTGTGGGCAAAATGATCGGCGGAATTGGACGGTTTTACATACAAATGTGTACCGAGCT
  GAAGCTGTCTGATTATGAGGGTAGGCTCATTCAGAACAGCTTGACTATAGAACGAATGGTCCTGTCTGCC
  TTTGATGAGAGAAGAAATAAGTACCTCGAAGAACATCCTTCAGCAGGGAAGGACCCTAAGAAGACAGGGG
  GTCCAATTTACCGCCGCGTCAACGGGAAGTGGATGAGAGAACTCATACTGTACGACAAGGAAGAGATTAG
  AAGAATCTGGAGACAAGCCAACAATGGTGACGATGCAACCGCCGGGCTCACCCACATGATGATCTGGCAT
  AGTAACCTCAACGATGCCACCTATCAGAGAACACGAGCTCTGGTCAGAACGGGGATGGACCCAAGGATGT
  GCTCCTTGATGCAGGGAAGTACCCTCCCAAGGAGAAGCGGGGCAGCTGGAGCCGCAGTGAAGGGCGTAGG
  TACAATGGTTATGGAGTTGGTGCGGATGATAAAGCGGGGAATCAACGACAGGAACTTTTGGCGAGGTGAA
  AATGGGCGCAAAACTCGAATTGCGTACGAGAGGATGTGTAATATCCTCAAGGGCAAATTTCAAACAGCTG
  CTCAAAAAGCGATGATGGATCAGGTACGCGAGAGTCGCGACCCCGGCAACGCCGAGTTTGAAGATCTCAC
  CTTCCTTGCACGGAGCGCCCTGATCCTGAGGGGATCTGTTGCCCACAAAAGCTGCCTTCCCGCTTGCGTT
  TACGGGCCAGCCGTCGCGTCTGGGTATGATTTTGAGAGGGAAGGCTATTCCCTGGTGGGCATCGACCCCT
  TCCGGTTGTTGCAGAACAGTCAGGTATACTCTCTCATCAGGCCTAACGAGAACCCCGCCCACAAGTCCCA
  GCTTGTGTGGATGGCCTGCCACTCCGCAGCATTTGAGGATCTCCGAGTGCTGTCATTCATCAAAGGCACG
  AAAGTCGTCCCACGAGGTAAGCTGTCCACTCGGGGAGTGCAGATTGCAAGCAATGAAAACATGGAGACAA
  TGGAAAGTTCAACCTTGGAACTGAGGTCCAGATACTGGGCAATCCGGACCAGGAGCGGGGGCAACACAAA
  CCAGCAGCGAGCTTCTGCCGGGCAAATAAGCATACAGCCTACCTTCTCAGTACAGCGCAACCTCCCATTC
  GACCGCACCACAGTCATGGCCGCATTTACTGGGAATACCGAAGGGAGGACCTCCGATATGAGGACCGAGA
  TAATTCGCATGATGGAAAGCGCTCGCCCTGAAGACGTGAGCTTTCAGGGGCGGGGAGTGTTCGAGCTGTC
  CGATGAAAAGGCCGCTTCTCCCATTGTTCCAAGCTTTGACATGTCCAACGAAGGGTCATACTTTTTTGGT
  GACAATGCCGAGGAATATGATAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102478 representing NP_040982
  Red=Cloning sites Green=Tags
  
  MASQGTKRSYEQMETDGERQNATEIRASVGKMIGGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSA
  FDERRNKYLEEHPSAGKDPKKTGGPIYRRVNGKWMRELILYDKEEIRRIWRQANNGDDATAGLTHMMIWH
  SNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELVRMIKRGINDRNFWRGE
  NGRKTRIAYERMCNILKGKFQTAAQKAMMDQVRESRDPGNAEFEDLTFLARSALILRGSVAHKSCLPACV
  YGPAVASGYDFEREGYSLVGIDPFRLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRVLSFIKGT
  KVVPRGKLSTRGVQIASNENMETMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISIQPTFSVQRNLPF
  DRTTVMAAFTGNTEGRTSDMRTEIIRMMESARPEDVSFQGRGVFELSDEKAASPIVPSFDMSNEGSYFFG
  DNAEEYDN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002019
• ORF Size: 1494 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002019.1, NP_040982
• RefSeq ORF: 1494
• Locus ID: 956531
• MW: 56.2 kDa