NS1 (NC_002211) Virus Tagged ORF Clone

CAT#: VC102487

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS-1 [Influenza B virus], codon optimized for human cell expression, NP_056666

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102487 represents NCBI reference of NP_056666 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGATAATATGACAACGACCCAGATAGAGGTCGGCCCAGGCGCCACAAATGCGACCATAAACTTCG
  AGGCTGGCATTCTGGAATGCTATGAGAGATTCAGCTGGCAAAGAGCCCTCGATTACCCAGGCCAGGACAG
  ACTCCACCGGCTGAAGCGGAAGTTGGAGTCTCGGATCAAGACCCATAATAAAAGCGAGCCTGAGAACAAG
  AGAATGAGCCTGGAAGAGAGGAAAGCCATCGGTGTGAAAATGATGAAGGTGCTCCTGTTCATGGACCCAT
  CTGCAGGTATAGAAGGCTTTGAGCCATACTGCGTGAAGAATCCGAGCACTAGCAAGTGTCCTAATTACGA
  CTGGACCGATTATCCCCCTACCCCTGGCAAGTATCTCGACGACATCGAAGAGGAACCCGAGAACGTGGAT
  CACCCTATCGAGGTAGTGTTGCGCGACATGAACAATAAAGATGCTCGACAGAAAATCAAGGATGAGGTCA
  ACACGCAAAAGGAAGGTAAGTTTAGGTTGACAATAAAACGCGACATCCGGAACGTGTTGTCTTTGCGGGT
  GCTGGTGAACGGGACATTTTTGAAACACCCTAACGGAGATAAGTCTCTTTCTACACTGCATCGACTGAAT
  GCCTACGACCAGAATGGGGGCTTGGTGGCCAAGCTCGTCGCAACGGACGACCGCACAGTTGAAGACGAAA
  AAGATGGCCATCGCATCCTTAACTCCCTGTTCGAACGATTTGACGAAGGGCACTCAAAGCCTATTCGCGC
  GGCCGAAACTGCTGTGGGCGTACTCAGCCAGTTTGGCCAGGAGCACAGACTTAGTCCCGAAGAAGGAGAC
  AAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102487 representing NP_056666
  Red=Cloning sites Green=Tags
  
  MADNMTTTQIEVGPGATNATINFEAGILECYERFSWQRALDYPGQDRLHRLKRKLESRIKTHNKSEPENK
  RMSLEERKAIGVKMMKVLLFMDPSAGIEGFEPYCVKNPSTSKCPNYDWTDYPPTPGKYLDDIEEEPENVD
  HPIEVVLRDMNNKDARQKIKDEVNTQKEGKFRLTIKRDIRNVLSLRVLVNGTFLKHPNGDKSLSTLHRLN
  AYDQNGGLVAKLVATDDRTVEDEKDGHRILNSLFERFDEGHSKPIRAAETAVGVLSQFGQEHRLSPEEGD
  N
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002211
• ORF Size: 843 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002211.1, NP_056666
• RefSeq ORF: 843
• Locus ID: 956544
• MW: 32.1 kDa