NP (NC_006311) Virus Tagged ORF Clone

CAT#: VC102495

Myc-DDK-tagged ORF clone of viral ORF for nucleoprotein [Influenza C virus (C/Ann Arbor/1/50)], codon optimized for human cell expression, YP_089656

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102495 represents NCBI reference of YP_089656 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGACCGGCGGCAAAATAGAAAGACCCCAGACGAACAGCGGAAGGCCAACGCACTGATCATCAACG
  AGAATATTGAGGCCTACATTGCCATTTGCAAAGAGGTGGGCCTCAACGGCGACGAGATGCTCATTCTGGA
  GAACGGCATTGCCATCGAGAAGGCCATCCGGATCTGCTGCGACGGCAAATACCAGGAAAAGCGGGAAAAG
  AAGGCTCGGGAGGCCCAACGGGCTGACTCCAACTTCAACGCCGACTCCATCGGCATTAGACTCGTGAAGC
  GGGCCGGCAGCGGCACCAACATCACCTACCACGCCGTGGTGGAACTGACCAGCCGGTCCAGAATCGTGCA
  GATCCTGAAAAGCCACTGGGGCAACGAGCTGAATCGGGCCAAGATCGCAGGGAAGCGGCTGGGCTTTAGC
  GCCCTCTTTGCCAGCAACCTGGAGGCCATCATCTACCAGCGGGGGCGGAATGCCGCCCGGCGGAATGGGT
  CCGCCGAGCTGTTCACCCTGACACAGGGCGCCGGAATCGAGACCCGGTACAAGTGGATCATGGAGAAGCA
  CATCGGGATCGGCGTGCTGATCGCCGACGCCAAGGGCCTCATCAACGGCAAGCGGGAGGGCAAACGGGGC
  GTCGATGCCAACGTCAAGCTGCGGGCCGGCACCACCGGCAGCCCACTGGAGCGGGCCATGCAGGGCATCG
  AGAAGAAGGCATTCCCCGGCCCACTGCGGGCCCTGGCCCGGCGGGTGGTCAAGGCCAACTACAACGACGC
  CCGGGAGGCCCTCAATGTCATCGCCGAGGCCAGCCTCCTGCTGAAGCCCCAGATCACCAACAAGATGACC
  ATGCCCTGGTGCATGTGGCTCGCCGCCCGGCTGACCCTCAAGGACGAGTTCGCCAACTTCTGCGCCTACG
  CCGGGCGGCGGGCCTTCGAGGTCTTCAACATCGCTATGGAGAAGATCGGCATTTGCAGCTTCCAGGGGAC
  CATCATGAACGACGACGAGATCGAAAGCATCGAGGACAAGGCACAGGTCCTCATGATGGCCTGCTTCGGC
  CTGGCCTATGAGGACTTCTCCCTGGTGAGCGCCATGGTGAGCCACCCACTCAAGCTGCGGAACCGGATGA
  AGATCGGCAACTTTCGGGTCGGCGAGAAGGTGAGCACCGTCCTCAGCCCCCTGCTGCGGTTCACCCGGTG
  GGCCGAGTTCGCCCAGCGGTTCGCCCTGCAGGCCAATACCTCCCGGGAGGGCGCCCAGATCAGCAACAGC
  GCCGTGTTCGCCGTGGAGCGGAAGATCACCACCGACGTGCAGCGGGTGGAGGAGCTGCTGAACAAGGTGC
  AGGCCCACGAGGACGAGCCTCTGCAGACCCTGTACAAGAAGGTGCGGGAGCAGATCAGCATCATCGGCCG
  GAACAAAAGCGAGATCAAGGAGTTCCTGGGCAGCAGCATGTACGACCTGAACGACCAGGAGAAGCAGAAC
  CCCATCAACTTTCGGAGCGGGGCCCACCCCTTTTTCTTCGAGTTCGATCCCGACTACAACCCAATCCGGG
  TCAAGCGGCCCAAGAAGCCCATCGCCAAGCGGAACAGCAACATCAGCCGGCTGGAAGAGGAGGGCATGGA
  CGAGAACAGCGAGATCGGACAGGCCAAGAAGATGAAGCCCCTCGACCAGCTCACCAGCACCAGCAGCAAT
  ATCCCCGGGAAAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102495 representing YP_089656
  Red=Cloning sites Green=Tags
  
  MSDRRQNRKTPDEQRKANALIINENIEAYIAICKEVGLNGDEMLILENGIAIEKAIRICCDGKYQEKREK
  KAREAQRADSNFNADSIGIRLVKRAGSGTNITYHAVVELTSRSRIVQILKSHWGNELNRAKIAGKRLGFS
  ALFASNLEAIIYQRGRNAARRNGSAELFTLTQGAGIETRYKWIMEKHIGIGVLIADAKGLINGKREGKRG
  VDANVKLRAGTTGSPLERAMQGIEKKAFPGPLRALARRVVKANYNDAREALNVIAEASLLLKPQITNKMT
  MPWCMWLAARLTLKDEFANFCAYAGRRAFEVFNIAMEKIGICSFQGTIMNDDEIESIEDKAQVLMMACFG
  LAYEDFSLVSAMVSHPLKLRNRMKIGNFRVGEKVSTVLSPLLRFTRWAEFAQRFALQANTSREGAQISNS
  AVFAVERKITTDVQRVEELLNKVQAHEDEPLQTLYKKVREQISIIGRNKSEIKEFLGSSMYDLNDQEKQN
  PINFRSGAHPFFFEFDPDYNPIRVKRPKKPIAKRNSNISRLEEEGMDENSEIGQAKKMKPLDQLTSTSSN
  IPGKN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006311
• ORF Size: 1695 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006311.1, YP_089656
• RefSeq ORF: 1695
• Locus ID: 3077360
• MW: 63.6 kDa