Dgkg (NM_138650) Mouse Tagged ORF Clone Lentiviral Particle

CAT#: MR210661L4V

Lenti ORF particles, Dgkg (GFP-tagged) - Mouse diacylglycerol kinase, gamma (Dgkg), 200ul, >10^7 TU/mL

Product Images

MR210661L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with MR210661L4V particle to overexpress human Dgkg-mGFP fusion protein.

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: mGFP
• Symbol: Dgkg
• Synonyms: 90kDa; 2900055E17Rik; AI854428; Dagk3; E430001K23Rik; mKIAA4131
• Mammalian Cell Selection: Puromycin
• Vector: pLenti-C-mGFP-P2A-Puro
• ACCN: NM_138650
• ORF Size: 2367 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(MR210661).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138650.1
• RefSeq Size: 5521 bp
• RefSeq ORF: 2367 bp
• Locus ID: 110197
• UniProt ID: Q91WG7
• Cytogenetics: 16 13.37 cM
• Gene Summary: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:32033984). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:32033984). Has no apparent specificity with regard to the acyl compositions of diacylglycerol (By similarity). Specifically expressed in the cerebellum where it controls the level of diacylglycerol which in turn regulates the activity of protein kinase C gamma (PubMed:32033984). Through protein kinase C gamma, indirectly regulates the dendritic development of Purkinje cells, cerebellar long term depression and ultimately cerebellar motor coordination (PubMed:32033984).[UniProtKB/Swiss-Prot Function]