FRK (NM_002031) Human Mutant ORF Clone

CAT#: RC402609

FRK Mutant (R239X), Myc-DDK-tagged ORF clone of Homo sapiens fyn-related kinase (FRK) as transfection-ready DNA

Specifications

Product Data

• Mutation Description: R239X
• Affected Codon#: 239
• Affected NT#: 715
• Tag: Myc-DDK
• Effect: Potential protein deficiency
• Symbol: FRK
• Synonyms: GTK; PTK5; RAK
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Vector: pCMV6-Entry
• ACCN: NM_002031
• ORF Size: 714 bp
• Sequence Data:
  ORF Nucleotide Sequence

  >RC402609 representing NM_002031
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAACATCTGTCAGAGGCTCTGGGAGTACCTAGAACCCTATCTCCCCTGTTTGTCCACGGAGGCAG
  ACAAGTCAACCGTGATTGAAAATCCAGGGGCCCTTTGCTCTCCCCAGTCACAGAGGCATGGCCACTACTT
  TGTGGCTTTGTTTGATTACCAGGCTCGGACTGCTGAGGACTTGAGCTTCCGAGCAGGTGACAAACTTCAA
  GTTCTGGACACTTTGCATGAGGGCTGGTGGTTTGCCAGACACTTGGAGAAAAGACGAGATGGCTCCAGTC
  AGCAACTACAAGGCTATATTCCTTCTAACTACGTGGCTGAGGACAGAAGCCTACAGGCAGAGCCGTGGTT
  CTTTGGAGCAATCGGAAGATCAGATGCAGAGAAACAACTATTATATTCAGAAAACAAGACCGGTTCCTTT
  CTAATCAGAGAAAGTGAAAGCCAAAAAGGAGAATTCTCTCTTTCAGTTTTAGATGGAGCAGTTGTAAAAC
  ACTACAGAATTAAAAGACTGGATGAAGGGGGATTTTTTCTCACGCGAAGAAGAATCTTTTCAACACTGAA
  CGAATTTGTGAGCCACTACACCAAGACAAGTGACGGCCTGTGTGTCAAGCTGGGGAAACCATGCTTAAAG
  ATCCAGGTCCCAGCTCCATTTGATTTGTCGTATAAAACCGTGGACCAATGGGAGATAGACCGCAACTCCA
  TACAGCTTCTGAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGA TAAGGTTTAA
  Protein Sequence

  >RC402609 representing NM_002031
  Red=Cloning site Green=Tags(s)
  
  MSNICQRLWEYLEPYLPCLSTEADKSTVIENPGALCSPQSQRHGHYFVALFDYQARTAEDLSFRAGDKLQ
  VLDTLHEGWWFARHLEKRRDGSSQQLQGYIPSNYVAEDRSLQAEPWFFGAIGRSDAEKQLLYSENKTGSF
  LIRESESQKGEFSLSVLDGAVVKHYRIKRLDEGGFFLTRRRIFSTLNEFVSHYTKTSDGLCVKLGKPCLK
  IQVPAPFDLSYKTVDQWEIDRNSIQLLK
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NP_002022
• RefSeq Size: 714
• RefSeq ORF: 1518
• Locus ID: 2444
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 26.2 kDa
• Gene Summary: The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008]