Human c Abl (ABL1) activation kit by CRISPRa

CAT#: GA100017

ABL1 CRISPRa kit - CRISPR gene activation of human ABL proto-oncogene 1, non-receptor tyrosine kinase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ABL1
• Locus ID: 25
• Kit Components:

  GA100017G1, c Abl gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100017G2, c Abl gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100017G3, c Abl gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_005157, NM_007313
• Synonyms: ABL; bcr/abl; c-ABL; c-ABL1; JTK7; p150; v-abl
• Summary: 'This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]'