Human alpha Actinin 4 (ACTN4) activation kit by CRISPRa

CAT#: GA100051

ACTN4 CRISPRa kit - CRISPR gene activation of human actinin alpha 4

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ACTN4
• Locus ID: 81
• Kit Components:

  GA100051G1, alpha Actinin 4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100051G2, alpha Actinin 4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100051G3, alpha Actinin 4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_004924, NM_001322033
• Synonyms: ACTININ-4; FSGS; FSGS1
• Summary: ' Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]'