Human Adenosine Receptor A2a (ADORA2A) activation kit by CRISPRa

CAT#: GA100094

ADORA2A CRISPRa kit - CRISPR gene activation of human adenosine A2a receptor

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ADORA2A
• Locus ID: 135
• Kit Components:

  GA100094G1, Adenosine Receptor A2a gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100094G2, Adenosine Receptor A2a gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100094G3, Adenosine Receptor A2a gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000675, NM_001278497, NM_001278498, NM_001278499, NM_001278500, NR_103543, NR_103544
• Synonyms: A2aR; ADORA2; RDC8
• Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]'