Human Aspartate beta hydroxylase (ASPH) activation kit by CRISPRa

CAT#: GA100320

ASPH CRISPRa kit - CRISPR gene activation of human aspartate beta-hydroxylase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ASPH
• Locus ID: 444
• Kit Components:

  GA100320G1, Aspartate beta hydroxylase gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100320G2, Aspartate beta hydroxylase gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100320G3, Aspartate beta hydroxylase gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001164750, NM_001164751, NM_001164752, NM_001164753, NM_001164754, NM_001164755, NM_001164756, NM_004318, NM_020164, NM_032466, NM_032467, NM_032468, N83868
• Synonyms: AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN; junctin
• Summary: 'This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis. [provided by RefSeq, Sep 2009]'