Human BCAT1 activation kit by CRISPRa

CAT#: GA100404

BCAT1 CRISPRa kit - CRISPR gene activation of human branched chain amino acid transaminase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: BCAT1
• Locus ID: 586
• Kit Components:

  GA100404G1, BCAT1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100404G2, BCAT1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100404G3, BCAT1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001178091, NM_001178092, NM_001178093, NM_001178094, NM_005504
• Synonyms: BCATC; BCT1; ECA39; MECA39; PNAS121; PP18
• Summary: 'This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]'