Human Carbonic Anhydrase I (CA1) activation kit by CRISPRa

CAT#: GA100527

CA1 CRISPRa kit - CRISPR gene activation of human carbonic anhydrase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CA1
• Locus ID: 759
• Kit Components:

  GA100527G1, Carbonic Anhydrase I gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100527G2, Carbonic Anhydrase I gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100527G3, Carbonic Anhydrase I gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001128829, NM_001128830, NM_001128831, NM_001164830, NM_001291967, NM_001291968, NM_001738, NM_001291968.2, NM_001291967.2
• Synonyms: CA-I; CAB; Car1; HEL-S-11
• Summary: 'Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Nov 2016]'