Human Carbonic Anhydrase IX (CA9) activation kit by CRISPRa

CAT#: GA100535

CA9 CRISPRa kit - CRISPR gene activation of human carbonic anhydrase 9

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CA9
• Locus ID: 768
• Kit Components:

  GA100535G1, Carbonic Anhydrase IX gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100535G2, Carbonic Anhydrase IX gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100535G3, Carbonic Anhydrase IX gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001216
• Synonyms: CAIX; MN
• Summary: 'Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]'