Human Collagen IV (COL4A3) activation kit by CRISPRa

CAT#: GA100899

COL4A3 CRISPRa kit - CRISPR gene activation of human collagen type IV alpha 3 chain

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: COL4A3
• Locus ID: 1285
• Kit Components:

  GA100899G1, Collagen IV gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100899G2, Collagen IV gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100899G3, Collagen IV gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000091, NM_031362, NM_031363, NM_031364, NM_031365, NM_031366
• Synonyms: alpha 3 type IV collagen; alpha3 type IV collagen; collagen, type IV, alpha 3 (Goodpasture antigen); collagen IV, alpha-3 polypeptide; Goodpasture antigen; OTTHUMP00000195044; TUMSTATIN; tumstatin
• Summary: 'Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]'