Human DGKG activation kit by CRISPRa

CAT#: GA101132

DGKG CRISPRa kit - CRISPR gene activation of human diacylglycerol kinase gamma

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DGKG
• Locus ID: 1608
• Kit Components:

  GA101132G1, DGKG gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101132G2, DGKG gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101132G3, DGKG gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001080744, NM_001080745, NM_001346
• Synonyms: DAGK3; DGK-GAMMA
• Summary: 'This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'