Human DNase I (DNASE1) activation kit by CRISPRa

CAT#: GA101232

DNASE1 CRISPRa kit - CRISPR gene activation of human deoxyribonuclease 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DNASE1
• Locus ID: 1773
• Kit Components:

  GA101232G1, DNase I gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101232G2, DNase I gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101232G3, DNase I gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_005223, NM_001351825
• Synonyms: DNL1; DRNI
• Summary: 'This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]'