Human Endothelin 3 (EDN3) activation kit by CRISPRa

CAT#: GA101329

EDN3 CRISPRa kit - CRISPR gene activation of human endothelin 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: EDN3
• Locus ID: 1908
• Kit Components:

  GA101329G1, Endothelin 3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101329G2, Endothelin 3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101329G3, Endothelin 3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000114, NM_001302455, NM_001302456, NM_207032, NM_207033, NM_207034
• Synonyms: ET-3; ET3; HSCR4; PPET3; WS4B
• Summary: 'The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]'