Human ERCC1 activation kit by CRISPRa

CAT#: GA101444

ERCC1 CRISPRa kit - CRISPR gene activation of human ERCC excision repair 1, endonuclease non-catalytic subunit

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ERCC1
• Locus ID: 2067
• Kit Components:

  GA101444G1, ERCC1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101444G2, ERCC1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101444G3, ERCC1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001166049, NM_001983, NM_202001, NM_001369409, NM_001369414, NM_001369416, NM_001369419, NM_001369408, NM_001369410, NM_001369411, NM_001369412, NM_001369413, NM_001369415, NM_001369417, NM_001369418
• Synonyms: COFS4; RAD10; UV20
• Summary: 'The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]'