Human GTF2H2 activation kit by CRISPRa

CAT#: GA102010

GTF2H2 CRISPRa kit - CRISPR gene activation of human general transcription factor IIH subunit 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: GTF2H2
• Locus ID: 2966
• Kit Components:

  GA102010G1, GTF2H2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102010G2, GTF2H2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102010G3, GTF2H2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001515, NM_001364567, NM_001364570, NM_001364568, NM_001364569, NM_001364571, NM_001364572, NM_001364573
• Synonyms: BTF2; BTF2P44; p44; T-BTF2P44; TFIIH
• Summary: 'This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however, no sequence data is currently available for the centromeric copy of the gene. [provided by RefSeq, Jul 2008]'