Human Heparan Sulfate Proteoglycan 2 (HSPG2) activation kit by CRISPRa

CAT#: GA102274

HSPG2 CRISPRa kit - CRISPR gene activation of human heparan sulfate proteoglycan 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: HSPG2
• Locus ID: 3339
• Kit Components:

  GA102274G1, Heparan Sulfate Proteoglycan 2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102274G2, Heparan Sulfate Proteoglycan 2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102274G3, Heparan Sulfate Proteoglycan 2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001291860, NM_005529
• Synonyms: HSPG; PLC; PRCAN; SJA; SJS; SJS1
• Summary: 'This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and transthyretin, etc., and it plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and tardive dyskinesia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]'