Human Myelin Basic Protein (MBP) activation kit by CRISPRa

CAT#: GA102833

MBP CRISPRa kit - CRISPR gene activation of human myelin basic protein

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: MBP
• Locus ID: 4155
• Kit Components:

  GA102833G1, Myelin Basic Protein gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102833G2, Myelin Basic Protein gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102833G3, Myelin Basic Protein gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001025081, NM_001025090, NM_001025092, NM_001025094, NM_001025098, NM_001025100, NM_001025101, NM_002385, N20370
• Synonyms: MGC99675
• Summary: 'The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]'