Human MEKK3 (MAP3K3) activation kit by CRISPRa

CAT#: GA102872

MAP3K3 CRISPRa kit - CRISPR gene activation of human mitogen-activated protein kinase kinase kinase 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: MAP3K3
• Locus ID: 4215
• Kit Components:

  GA102872G1, MAP3K3 gRNA vector 1 in pCas-Guide-CRISPRa

  GA102872G2, MAP3K3 gRNA vector 2 in pCas-Guide-CRISPRa

  GA102872G3, MAP3K3 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001330431, NM_002401, NM_203351, NM_001363768
• Synonyms: MAPKKK3; MEKK3
• Summary: This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]