Human PCCA activation kit by CRISPRa

CAT#: GA103406

PCCA CRISPRa kit - CRISPR gene activation of human propionyl-CoA carboxylase subunit alpha

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PCCA
• Locus ID: 5095
• Kit Components:

  GA103406G1, PCCA gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103406G2, PCCA gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103406G3, PCCA gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000282, NM_001127692, NM_001178004, NM_001352605, NM_001352606, NM_001352607, NM_001352608, NM_001352609, NM_001352610, NM_001352611, NM_001352612, NR_148027, NR_148028, NR_148029, NR_148030, NR_148031
• Synonyms: PCCase alpha subunit; propanoyl-CoA:carbon dioxide ligase alpha subunit; propionyl-CoA carboxylase alpha chain, mitochondrial; propionyl-coenzyme A carboxylase, alpha polypeptide; Propionyl Coenzyme A carboxylase alpha polypeptide
• Summary: 'The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]'