Human PDE1A activation kit by CRISPRa

CAT#: GA103433

PDE1A CRISPRa kit - CRISPR gene activation of human phosphodiesterase 1A

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PDE1A
• Locus ID: 5136
• Kit Components:

  GA103433G1, PDE1A gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103433G2, PDE1A gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103433G3, PDE1A gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001003683, NM_001258312, NM_001258313, NM_001258314, NM_005019, NM_001363871
• Synonyms: CAM-PDE-1A; HCAM-1; HCAM1; HSPDE1A
• Summary: 'Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]'